Quality of fresh milt of grey mullet Mugil cephalus Linnaeus, 1758 was assessed based on motility grade, motility duration, sperm count and seminal plasma osmolality. Freshly collected milt from live males (n=17) were examined individually for motility assessment. Motility grade was four in fifteen samples whereas three in other two samples analysed. Other sperm quality parameters such as motil...

INTRODUCTION The solution in which graft tissue is stored (that is, preservation solution) is an important component of liver transplantation technology. Its protective effect is induced by substances in the solution, including radical scavengers, buffers, and energy-giving substances. New preservation solutions have proven to be effective in preventing organ damage during cold ischemia and in ...

BACKGROUND The question of whether the choice of preservation solution affects outcome after liver transplantation is still not satisfactorily answered. The purpose of this study is to examine the preservation solutions' impact on outcome after liver transplantation. METHODS A double-center retrospective study of short- and long-term results of 3134 consecutive liver transplantations with fol...

With the advent of data-driven science, also referred to as, for example, the Fourth Paradigm, Big Data, and other similar concepts, the need to safeguard the investments made into collecting and preparing massive amounts of data (some of which is unrecoverable) has drastically gained importance. Providing digital preservation of research data is thus emerging as a service that has to be provid...

The effects of different extenders and storage periods on the motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) semen were evaluated after short-term storage. Semen was collected from anesthetized males by the abdominal massage. After determination of main semen characteristics, the pooled ejaculates were diluted with 4 different extenders at a ratio of 1:3, and stored at 4...

Trehalose-glycerol mixtures are known to be effective in the long time preservation of proteins. However, the microscopic mechanism of their effective preservation abilities remains unclear. In this article we present a molecular dynamics simulation study of the short time, less than 1 ns, dynamics of four trehalose-glycerol mixtures at temperatures below the glass transition temperature. We fo...

The amount of content from digital origin permanently increases. The short lifespan of digital media makes it necessary to develop strategies to preserve its content for future use. Not only electronic documents, pictures and movies have to be preserved, also interactive content like digital art or video games have to be kept “alive” for future generations. In this paper we discuss strategies f...

Background: The availability of fresh stool samples is a prerequisite in most gut microbiota functional studies. Objective: Strategies for amplification and long-term gut microbiota preservation from fecal samples would favor sample sharing, help comparisons and reproducibility over time and between laboratories, and improve the safety and ethical issues surrounding fecal microbiota transplanta...