Instrumentation: Nuclear magnetic resonance (NMR) spectra were recorded on a BRUKER Ascend500 spectrometer (1H: 500 MHz and 13C: 126 MHz) at ambient temperature unless otherwise noted. Chemical shift values for protons were referenced to the residual proton resonance of chloroform-d (δ: 7.26). Chemical shift values for carbons were referenced to the carbon resonance of chloroform-d (δ: 77.16). ...

This hospital was established in September 1971, and is currently a university hospital comprising two basement levels and 19 aboveground floors (Fig. 1). With its attached critical care center and general perinatal medical center as well as its 23 medical departments offering 1154 beds, it contributes to regional health services and functions as an advanced treatment hospital. The hospital han...

The purpose of this study was to monitor the concentrations of mercury in the edible muscle, gill, liver, and skin of common carp (Cyprinus carpio) and silver carp (Hypophthalmichthys molitrix), in the Sanandaj Gheshlagh Reservoir, Iran. Mercury concentrations were assayed using Shimadzu AA 6600 atomic absorption spectrophotometer, and the results were given as µg/g wet weight. The level of mer...

Current research paper describes highly specific and reproducible 1 order derivative spectroscopic method for quantitative analysis of Sitagliptin which is a DPP4 inhibitors Dapagliflozin SGLT2 from its synthetic mixture. Both drugs are Anti Diabetics class. Present analytical was developed on Shimadzu double beam spectrophotometer equipped with UV probe 2.42 as software using methyl alcohol so...

A simple, rapid and precise reverse phase high performance liquid chromatography method has been developed and validated for the determination of Afloqualone in tablet and serum. Chromatography was performed by Shimadzu Model LC-20 ATVP with Kromasil C-18 column 250 x 4.6 mm ID with 5  particle sizes and the column maintained at ambient temperature. The injection volume was 20l and the total ...

To elucidate the functions of phospholipids, it is important to conduct analysis not only of their classes and sub-classes, but of their molecular species as well. At Shimadzu, we found that electrospray ionization (ESI) MS3 analysis is effective for more detailed and accurate annotation of each molecular species. We established a system for phospholipid molecular species analysis using a neutr...

An automated headspace gas-chromatographic method has been developed for the determination of acetone in the milk, blood and urine of dairy cows. Five ml samples were saturated with 2 g of sodium chloride and equilibrated for 30 min at 90 degrees C in a Hewlett-Packard HP 19395 A automatic headspace sampler. The headspace volatiles were transferred without splitting to a 25 m x 0.3 mm x 0.4 mic...

Gabapentin is an anticonvulsant widely used in the treatment of epilepsy.A simple, rapid, accurate, precise and economic spectrofluorimetric methods have been developed for the estimation of gabapentin in pharmaceutical formulations and in spiked human plasma. The analysis and validation dine by using SHIMADZU RF – 5301 Spectofluorimeter, equipped with Xenon arc lamp, using quartz cell (1×1×4.5...