BACKGROUND Shigella species are among the common causes of bacterial diarrhoeal diseases. Traditional detection methods are time-consuming resulting in delay in treatment and control of Shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of Shigella spp. In this study a rapid multiplex PCR were developed for simultaneous detection of three ...

It has been shown that phase variation of Sh. sonnei is accompanied by changes in morphology and antigenic structure. Two mutants of the Phase I organism (II(S) and II(R)), which were studied, elaborate somatic antigens which are immunologically identical. The purified lipocarbohydrate-protein antigens of the Phase I and II(S) microorganisms are chemically similar yet immunologically distinct a...

The colicin typing method for differentiating epidemic strains of Shigella sonnei was evaluated. Forty percent of the strains were untypable, but the method divides 60% of the strains into 15 established colicin types. The method has reasonably good reproducibility, but should not be interpreted with the same degree of confidence as some other characteristics such as serotypes. For this reason,...

the colicine types, biotypes and resistance pattern to antibacterial drugs of 154 strains of shigella sonnei isolated in two different geographical areas of iran, are presented. while a difference in the predominant biotype was observed in the two areas, the resistance pattern and the colicine types were found to be almost identical.

To characterize Shigella clinical strains, we studied 82 Shigella strains recovered from 719 stool samples of patients with bloody diarrhea in Shiraz, Iran, over the period from April to October 2003. Serological assay classified the Shigella isolates as follows: 61 (74.39%) Shigella sonnei isolates, 16 (19.51%) Shigella flexneri isolates, 3 (3.65%) Shigella boydii isolates, and 2 (2.43%) Shige...

A Shiga toxin (Stx)-encoding temperate bacteriophage of Shigella sonnei strain CB7888 was investigated for its morphology, DNA similarity, host range, and lysogenization in Shigella and Escherichia coli strains. Phage 7888 formed plaques on a broad spectrum of Shigella strains belonging to different species and serotypes, including Stx-producing Shigella dysenteriae type 1. With E. coli, only s...

Smooth strains of Shigella dysenteriae type 1, Shigella flexneri, Shigella boydii, and Shigella sonnei which form pigmented colonies (Pcr+) on Congo red agar were virulent in the Sereny test. Smooth variants unable to bind Congo red (Pcr-) were avirulent. Measurements of dye uptake from solution showed that S. dysenteriae type 1 bound the most dye, followed in order of uptake by S. flexneri, S....

Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains (n=152) were isolated from foods and...

The plasmid-borne mph(A) gene that confers resistance to azithromycin and has recently emerged in Shigella sonnei is present in multidrug- and non-multidrug-resistant Escherichia coli isolates from 4 continents. Further spread of mph(A) to Shigella and Salmonella spp. may be expected.