BACKGROUND Up to a 20-fold variation in serum cardiac troponin I (cTnI) concentration may be observed for a given patient sample with different analytical methods. Because more limited variation is seen for control materials and for purified cTnI, we explored the possibility that cTnI was present in altered forms in serum. METHODS We used four recombinantly engineered cTnI fragments to study ...

Standardization of cardiac troponin I (cTnI) measurement is important because of the central role for diagnosis of myocardial infarction. In blood, cTnI is present as a heterogeneous mixture of different molecular species. The analytical problem caused by this heterogeneity may be circumvented by recognition of a unique, invariant part of the molecule that is common to all components of the mix...

BACKGROUND: Reliability of cardiac troponin-I assays under real-time conditions has not been previously well studied. Most large published cTnI trials have utilized protocols which required the freezing of serum (or plasma) for delayed batch cTnI analysis. We sought to correlate the presence of the acute ischemic coronary syndrome (AICS) to troponin-I values obtained in real-time by three rando...

We have investigated the binding of bepridil to calcium-saturated cardiac troponin C in a cardiac troponin C/troponin I complex. Nuclear magnetic resonance spectroscopy and [(15)N,(2)H]cardiac troponin C permitted the mapping of bepridil-induced amide proton chemical shifts. A single bepridil-binding site in the regulatory domain was found with an affinity constant of approximately 140 microM(-...

BACKGROUND: Cardiac Troponin I (cTnI) is known as a cardiac biomarker in determining the myocardial damage of diseases which affect the heart muscle. OBJECTIVES: This study aims to evaluate the serum cTnI concentration in Markhoz Breed goats suffering selenium (Se) deficiency and its correlation with electrocardiographic parameters, and activity of creatine kinase (CK) and aspartate aminotransf...

objective(s):biochemical markers are important for the timely diagnosis and follow-up of ischemic events. most of the markers have been previously studied in the context of cardiac ischemia. however, research on markers of non-cardiac events has been insufficient. therefore, we investigated the relationship between troponin and myoglobin which are commonly used markers of cardiac ischemia, in n...

background polypeptide hormone leptin suppresses inflammation in the heart muscle and protects heart from diseases. the purpose of this study is to evaluate the relationship between leptin and troponin serum levels with cardiac involvement in patients with major beta thalassemia. materials and methods in this cross-sectional study, 70 children with major thalassemia were selected. two ml blood ...