In order to study cellular metabolism of low density lipoproteins (LDL), ultracentrifugal methods have been used to isolate the lipoproteins. The use of vertical rotor ultracentrifugation very quickly produces small quantities of diluted lipoproteins per gradient, as well as small volumes of lipoprotein-deficient serum. We present modifications to this method in order to prepare routinely more ...

Serum lipoproteins may enter the airways and appear in sputum (chyloptysis) when the lymphatic circulation is impaired by inflammation, neoplasia, or an abnormal proliferation of smooth muscle cells. While analyzing the bronchoalveolar lavage fluid of a patient with chyloptysis, we noticed that surfactant could not be separated from contaminating serum lipoproteins and speculated that lipoprote...

The demonstration of a role for serum lipoproteins in lipid transport in health and disease has stimulated interest in the biological activity of the lipid and protein moieties of these substances. For these investigations the development of improved methods for separating the lipids from whole serum or from the serum lipoproteins themselves is important but fully acceptable methods are not yet...

The aim of the present study was to determine whether there was any correlation between serum thyroid hormones, lipids, lipoproteins and the activities of erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GPX) in adult nonpregnant uniparous Iranian pure bred fat-tailed ewes. Blood samples (10 mL) were taken from the jugular vein of 30 clinically healthy Iranian adult nonpregnan...

Dyslipidemia has been associated with premature atherosclerosis and other pathological conditions, and the determination of serum lipids is of great importance in health and disease. Most of the lipids are transported in plasma in the form of lipoproteins. Four classes of lipoproteins have been recognized by electrophoresis: chylomicrons, beta-, pre-beta-, and alpha-lipoproteins. Fredrickson, L...