Use of serum-free media and the related genera of animal-free and protein-free media has increased significantly over the past 15 years. This is particularly true in industrial applications, in which the use of serum presents a safety hazard and a source of unwanted contamination in the production of biopharmaceuticals (1). As the name suggests, serum-free media are prepared without animal seru...

the purpose of this study was to examine the potential application of sorbitol, sucrose and gelatin in combination with glycerol in order to theileria annulata infected cell line cryo-preservation. the efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. as a result, the...

Embryonic rat cells cultured in a synthetic medium are stopped in G t phase if serum is omitted from the culture medium. On addition of calf serum these cells resume DNA synthesis after a lag phase of 15 — 20 hours. In the present paper the events concerning the metabolism of adenosine phosphates after stimulation by serum have been examined. The results are the following: 1. The concentration ...

Human intestinal Caco-2 cells were differentiated using serum-reduced medium with fetal bovine serum (FBS) added only to the basolateral (BL) medium, and four serum-free media, containing insulin, transferrin, selenium (ITS), or MITO+™ serum extender (ITS plus growth factors), with or without addition of a lipid mixture, respectively. Differentiation was assessed by monitoring monolayer permeab...

The regulation of lipid synthesis from acetate in cultures of L cells in response to changes in exogenous lipid supply has been studied, and the enzyme acetyl-CoA synthetase has been investigated in reference to its possible role in the regulation of lipid biosynthesis from acetate. When serum lipid was removed from monolayers of L cells, a relatively rapid stimulation of [14C]acetate incorpora...

Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

The PC12 clone is a noradrenergic cell line derived from a rat pheochromocytoma. In culture medium containing horse serum, PC12 cells undergo mitosis; when nerve growth factor (NGF) is included in the medium, the cells cease multiplication and extend neuritis. It is shown here: (a) that PC12 cells are not viable in serum-free medium. When serum is withdrawn, 90 percent of the cells die within 4...

Clinical expansion of mesenchymal stem cells (MSCs) is hampered by the lack of knowledge regarding how to prevent MSC apoptosis and promote their proliferation in serum-free medium. Our in vitro studies demonstrated that human umbilical cord MSCs (HUCMSCs) underwent apoptosis in the serum-free medium. When HUCMSCs were co-cultured with retinal pigment epithelial cells (ARPE19), however, HUCMSCs...

The authors successfully cultivated FM3A cell line in two kinds of serum free media, which was derived from a case of spontaneous mammary carcinoma converted in vivo into ascites form of C3H stock mice and was grown in a serum containing modified Eagle's medium for about seven months. Bovine plasma albumin, bactopeptone, sodium pyruvate and ferric chloride were effective as additives to the ser...