Protein kinase D (PKD), a serine/threonine kinase with emerging cardiovascular functions, phosphorylates cardiac troponin I (cTnI) at Ser(22)/Ser(23), reduces myofilament Ca(2+) sensitivity, and accelerates cross-bridge cycle kinetics. Whether PKD regulates cardiac myofilament function entirely through cTnI phosphorylation at Ser(22)/Ser(23) remains to be established. To determine the role of c...

Speech Emotion Recognition (SER) is a new and challenging research area with a wide range of applications in man-machine interactions. The aim of a SER system is to recognize human emotion by analyzing the acoustics of speech sound. In this study, we propose Spectral Pattern features (SPs) and Harmonic Energy features (HEs) for emotion recognition. These features extracted from the spectrogram ...

2 Ser Asn Asn Asn 14 Leu Ile Leu Leu 22 Ser Ser Leu Leu 31 Val Asp Val Val 67 Thr Ala Thr Ala 116 Val Ile Ile Ile 183 Ala Ala Glu Glu 210 Ala Ser Ser Ser 233 Val Ala Ala Ala 262 Thr Ile Thr Thr 277 Gln Gln Arg Gln 294 Thr Val Val Val 296 Pro Ser Ser Ser 305 Ala Ser Ser Ser 314 Ile Val Val Val 317 Glu Lys Lys Lys 378 Ile Leu Leu Leu 415 Leu Val Val Val 417 Asp Gly Gly Gly 421 Val Ala Ala Ala 432...

MAPKAP kinase 2 (MK2) is required for tumor necrosis factor synthesis. Tristetraprolin (TTP) binds to the 3'-untranslated region of tumor necrosis factor mRNA and regulates its fate. We identified in vitro and in vivo phosphorylation sites in TTP using nanoflow high pressure liquid chromatography microelectrospray ionization tandem mass spectrometry and novel methods for direct digestion of TTP...

hf level of ab initio calculations with basis-set 6-31g including full counterpoise correction hasbeen applied to compute the asphis potential with the ser and hisser potential with the asp inasphisser trimer. the potential energy surface has a minimum of -16.765 kcal/mol in r1=1.912nm and r2=2.719 nm. the optimum computed curves for two interactions were fitted withintermolecular pair potentia...