Results: Serum samples became PCR-positive before semen samples. During the first 6 days after inoculation, serum was PRRSV-positive in 36 of 40 boars, and semen was PRRSV-positive in four of 40 boars. Median time to detection was 36 and 48 hours for nested PCR and Taqman PCR, respectively. Results were inconsistent when a positive semen sample was pooled with negative semen. Elevated rectal te...

Subfertility problems are encountered frequently in the cattle and buffalo bulls commercially maintained for semen production in dairy farms and under field conditions for natural insemination. Reports are scarce on the incidence of subfertility in breeding bulls, especially in India. The objective of the present study was to assess the incidence of the male reproductive anomalies leading to di...

the effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°c was evaluated. semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body weights of 64 kg. semen samples were pooled and divided into 4 parts. each part was diluted with...

OBJECTIVE To compare cryosurvival rates between remote collections with NextGen kit (offsite) and onsite collection of semen samples from infertile men and those with cancer. METHODS Prefreeze and post-thaw sperm motility, total motile sperm, and percent cryosurvival rates were compared between samples collected from infertile men onsite at the Andrology Center (n = 10) and samples collected ...

There is much interest in the United States and other countries by dog breeders, pet owners, veterinarians and research workers who realize the great potential for the use of frozen semen. This potential is comparable to that already achieved in the cattle breeding industry. Since the first successful pregnancy utilizing frozen dog semen (Seager, 1969), work on aspects of the freezing of dog se...

AIM The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in Rusa timorensis. MATERIALS AND METHODS Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks inter...

Ejaculation was pharmacologically induced in a 13-year-old Quarter Horse stallion with a spiral fracture of the radius. The owners desired to have semen from the stallion frozen prior to euthanatizing the horse, but because of the debilitating injury, standard methods of semen collection could not be used. With the stallion standing quietly in a stall, a plastic collection bag was positioned ov...

The preservation of spermatozoa is an important tool used in conservation programs to increase the genetic diversity of threatened and endangered species. Although routinely used to manage conservation programs for higher vertebrates, there have been limited attempts to establish reproductive assistance programs for tortoises. The purpose of this study was to develop a model for collecting and ...