with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...

The efferent limb of secretory immunity depends on local production and selective epithelial transport of polymeric immunoglobulins (pIg). To this end there is a fascinating cooperation of the B-cell system and the epithelium with their production of two key factors, the J chain and the secretory component (SC), respectively. Interactions between activated leucocytes for example, T cells and ma...

  With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...

Secretory vesic1es from adrenal medulla contain catecholamines, nuc1eotides and proteins, all of which are released into the extracellular fluid during exocytosis. Adrenal medullary secretory vesic1es also contain high concentrations of Ca'+ [1]. The mechanism of the aecumulation of Ca 2+ into the vesicles is largely unknown and the experimental data eoncerning the uptake of Ca'+ into isolated ...

recombinant protein production in e. coli has several advantages over other expression systems. misfolding, inclusion body formation, and lack of eukaryotic post translational modification are the most disadvantages of this system. exporting of correctly folded proteins to the outside of reductive cytoplasmic environment through twin-arginine system could help to pass these limiting steps. two ...

The secretory pathway in plants mediates the transport of cargo molecules from their site of synthesis to other compartments within the pathway as well as to the cell wall. At the centre of this system is the Golgi apparatus, a transport hub that is responsible for receiving, modifying and sorting proteins for transport to their destinations. Several recent publications have investigated the ro...