Net HCO transport in the rabbit kidney cortical collecting duct (CCD) is mediated by simultaneous H 1 secretion and HCO secretion, most likely occurring in a and b -intercalated cells (ICs), respectively. The polarity of net HCO transport is shifted from secretion to absorption after metabolic acidosis or acid incubation of the CCD. We investigated this adaptation by measuring net HCO flux befo...

Atrial natriuretic peptide (ANP) levels correlate with hyperglycemia in diabetes mellitus, but ANP effects on pancreatic islet β-cell insulin secretion are controversial. ANP was investigated for short- and long-term effects on insulin secretion and mechanisms regulating secretion in isolated rat pancreatic islets. A 3-h incubation with ANP did not affect basal or glucose-stimulated islet insul...

BACKGROUND Sweet taste receptor is expressed in the taste buds and enteroendocrine cells acting as a sugar sensor. We investigated the expression and function of the sweet taste receptor in MIN6 cells and mouse islets. METHODOLOGY/PRINCIPAL FINDINGS The expression of the sweet taste receptor was determined by RT-PCR and immunohistochemistry. Changes in cytoplasmic Ca(2+) ([Ca(2+)](c)) and cAM...

AIMS/HYPOTHESIS Changes in cellular cholesterol level may contribute to beta cell dysfunction. Islets from low density lipoprotein receptor knockout (LDLR(-/-)) mice have higher cholesterol content and secrete less insulin than wild-type (WT) mice. Here, we investigated the association between cholesterol content, insulin secretion and Ca(2+) handling in these islets. METHODS Isolated islets ...

Enteric bacterial pathogens often increase intestinal Cl- secretion. Enteropathogenic Escherichia coli(EPEC) does not stimulate active ion secretion. In fact, EPEC infection decreases net ion transport in response to classic secretagogues. This has been presumed to reflect diminished Cl- secretion. The aim of this study was to investigate the influence of EPEC infection on specific intestinal e...

Abstract A continuous-flow Anaerobic/Anoxic/Oxic (A2/O) system was operated at different organic concentrations to systematically investigate the effect on nutrient removal, secretion characteristics of extracellular polymer, phosphorus forms transformation and changes in functional flora this system. The results showed that high loading more conducive promote polymeric substance (EPS), increas...

31 Anion secretion by colonic epithelium is dependent on apical CFTR-mediated anion 32 conductance and basolateral ion transport. In many tissues, the NKCC1 Na-K-2Cl 33 cotransporter mediates basolateral Cl uptake. However, additional evidence suggests that the 34 AE2 Cl/HCO3 exchanger, when coupled with the NHE1 Na/H exchanger or a Na-HCO3 35 cotransporter (NBC), contributes to HCO3 and/or Cl ...

Anion secretion by colonic epithelium is dependent on apical CFTR-mediated anion conductance and basolateral ion transport. In many tissues, the NKCC1 Na(+)-K(+)-2Cl(-) cotransporter mediates basolateral Cl(-) uptake. However, additional evidence suggests that the AE2 Cl(-)/HCO(3)(-) exchanger, when coupled with the NHE1 Na(+)/H(+) exchanger or a Na(+)-HCO(3)(-) cotransporter (NBC), contributes...