In this study, we proposed an improved algorithm for identifying proteins relevant to cancer. The algorithm was named two-layer molecular similarity ensemble approach (TL-SEA). We applied TL-SEA to analyzing the correlation between anticancer compounds (against cell lines K562, MCF7 and A549) and active compounds against separate target proteins listed in BindingDB. Several associations between...

A 64-channel array coil for magnetic resonance imaging (MRI) has been designed and constructed. The coil was built to enable the testing of a new imaging method, single echo acquisition (SEA) MRI, in which an independent full image is acquired with every echo. This is accomplished by entirely eliminating phase encoding and instead using the spatial information obtained from an array of very nar...

In order to solve many tasks of biotechnology, constant lines of the cells of marine invertebrates with a high growth potential are required, which are absent at present. We used the universal activator of transcription gal4 to change the degree of expression of genes of growth factors in embryonic sea urchin cells and, thereby, increase their proliferative activity. The fertilized sea urchin e...

AIM To construct the expression vector of B3 (scdsFv)-SEA (D227A) and to identify its binding and cytotoxic ability to B3 antigen positive carcinoma cell lines. METHODS This fusion protein was produced by a bacterial expression system in this study. It was expressed mainly in the inclusion body. The gene product was solubilized by guanidine hydrochloride, refolded by conventional dilution met...

The Marmara Sea is situated between the world’s largest permanently anoxic basin, the Black Sea, and an enclosed marginal sea, the Aegean Sea, which experienced quasi-periodic sapropel deposition since Miocene time. It is connected to the Black Sea and the Aegean Sea through the Straits of Bosphorus and Dardanelles, respectively. Sapropel M1, which contains from 1 to 2% total organic carbon, wa...

TCK, the flagellar creatine kinase (ATP:creatine N-phosphotransferase) of sperm from the sea urchin Strongylocentrotus purpuratus is a membrane-associated lipophilic protein involved in energy transport. The cDNA derived protein sequence contains a consensus site sufficient for the covalent attachment of myristate. To examine whether TCK was myristoylated, mouse fibroblast Swiss 3T3 and baby ha...