AIMS: Cardiac dysfunction is a complication of sepsis and contributes to morbidity and mortality. Since raising plasma apolipoprotein (apo) A-I and high density lipoprotein (HDL) concentration reduces sepsis complications, we tested the hypothesis that the apoA-I mimetic peptide 4F confers similar protective effects in rats treated with lipopolysaccharide (LPS). METHODS AND RESULTS: Male Sprag...

Protein quality in soybean seeds is strongly influenced by nutritional conditions. Glycinin (11S globulin) and β-conglycinin (7S globulin) are the 2 main proteins in soybean seeds. Protein quality of glycinin (11S) is higher than that of β-conglycinin (7S), due to the presence of higher amounts of s-containing amino acids (methionine and cysteine) in glycinin than β-conglycinin. Of the amino ac...

Genetic variability and relationships among ten rice varieties were studied by using 20 decamer random primers and SDSPAGE methods. Out of 22, 20 Random Amplified Polymorphic DNA (RAPD) primers revealed polymorphism, while the remaining 2 primers showed no reaction. The primers produced a total of 109 bands, of which 62 (56.88%) were polymorphic. The number of polymorphic fragments for each pri...

Name: Xanthomonas translucens pv. translucens (Jones et al.) Vauterin et al. Synonyms: Xanthomonas campestris pv. translucens (Jones et al.) Dye Xanthomonas campestris pv. cerealis (Hagborg) Dye Xanthomonas campestris pv. hordei (Hagborg) Dye Xanthomonas campestris pv. secalis (Reddy et al.) Dye Xanthomonas campestris pv. undulosa (Smith et al.) Dye Taxonomic position: Bacteria: Gracilicutes Co...

A novel mechanism of molecular disease was uncovered in a patient with prolonged thrombin time and a mild bleeding tendency. DNA sequencing of the fibrinogen A a chain indicated heterozygosity for a mutation of 20 Val -+ Asp. The molar ratio of fibrinopeptide A to B released by thrombin was substantially reduced at 0.64 suggesting either impaired cleavage or that the majority of the variant a-c...

The present study was carried out to study the national haemorrhagic septicaemia vaccine strains at their molecular level. The vaccine is bivalent contain Pasteurella multocida serotype E:2 and B:2. The vaccine strains were obtained from Central Veterinary Research Laboratory, Department of Biological Product, Soba. Culture characteristic and colonial morphology was ascertained in common labora...

Leuconostoc mesenteroides NRRL B-1149 produces extracellular dextransucrase which in this study was purified using different concentrations of polyethylene glycol (PEG). The dextran produced by this enzyme is unique in that it contains α(16) and α-(13) linkages which have clinical applications. The cell free supernatant with 0.9 U/mg enzyme specific activity was subjected to fractionation by ...

A quantitative urea-based amyloid b (Ab)-sodium dodecyl sulfate-polyacrylamide gel electrophoresis with Western immunoblot (Ab-SDS-PAGE/immunoblot) reveals highly conserved and disease-specific Ab peptide patterns (Ab 1-37, 1-38, 1-39, 1-40, 1-42) in Alzheimer’sdisease (AD)patientsandnondementedcontrols. For further standardization of this method, we analyzed cerebrospinal fluid (CSF) of eight ...

The occurrence of calreticulin, the main Ca21 binding protein in the endoplasmic reticulum of eukaryotic cells, was investigated in the unicellular green alga Chlamydomonas reinhardtii Dangeard. The biochemical characterization of a diethylaminoethyl purified extract highlighted the presence, on SDSPAGE, of a 55-kDa protein that stained blue with the Stains All dye, a diagnostic feature of acid...

Background and aims: Iron is stored in hepatocytes in the form of ferritin and haemosiderin. There is a marked increase in iron rich haemosiderin in iron overloaded livers, and ferric iron in amounts exceeding the ferritin and haemosiderin binding capacity may promote free radical generation, causing cellular damage. The aim of this study was to characterise hepatic haemosiderin using four anti...