We have used a "two-way pseudo-testcross" mapping strategy in combination with the random amplified polymorphic DNA (RAPD) assay to construct two moderate density genetic linkage maps for species of Eucalyptus. In the cross between two heterozygous individuals many single-dose RAPD markers will be heterozygous in one parent, null in the other and therefore segregate 1:1 in their F1 progeny foll...

Bacteria isolated from marine sponges found off the coast of Rio de Janeiro, Brazil, were screened for the production of antimicrobial substances. We report a new Pseudomonas putida strain (designated P. putida Mm3) isolated from the sponge Mycale microsigmatosa that produces a powerful antimicrobial substance active against multidrug-resistant bacteria. P. putida Mm3 was identified on the basi...

Genetic analysis in 53 early introduced clones of rubber tree (Hevea brasiliensis) collected from different areas in Southern Thailand was performed using RAPD (Random Amplified Polymorphic DNA) and microsatellite markers. Seventeen cultivated clones (34 samples) were also included to compare DNA patterns. DNA was isolated from leaf samples using CTAB buffer. One hundred and ninety two 10-base ...

The variation in two Bruguiera species in populations from different climatic and macrogeographic regions along the western to southeastern coastal area in Sri Lanka was studied using RAPD (Random Amplified Polymorphic DNA). Intraand interspecific variation was observed using 20 primers of the 45 primers screened. Some primers showed diagnostic banding patterns and allowed unambiguous different...

Visceral leishmaniasis (VL) is mainly due to the Leishmania donovani complex. VL is endemic in many countries worldwide including East Africa and the Mediterranean region where the epidemiology is complex. Taxonomy of these pathogens is under controversy but there is a correlation between their genetic diversity and geographical origin. With steady increase in genome knowledge, RAPD is still a ...

The Bacillus subtilis genome encodes eleven Rap proteins, which are conserved tetratricopeptide-containing regulatory proteins. Of those characterized to date, all except RapI negatively regulate response regulators, including Spo0F, ComA and DegU, via protein-protein interactions. RapD has not yet been fully characterized. It was examined whether RapD inhibits the expression of spoIIE, srfA an...

the effect of sub culturing frequencies and different cytokinins (benzyl amino purine (bap), 2-isopentenyl adenine (2ip) on genetic stability of micropropagated shoots from olive plant were investigated by using physiological traits and random amplified polymorphic dna (rapd) markers. axillary buds of the olive (cv. dezful) plants were cultured and subcultured in dkw medium, supplemented with b...

background: dogs have been previously reported to be reservoirs of leishmania infantum as the etiological agent of human visceral leishmaniasis in iran. we report a case of canine visceral leishmaniasis (vl) caused by l. tropica from the north- west of iran where human visceral leishmaniasis is endemic. methods: the canine vl was initially screened by dipstick rk39 and direct agglutination test...

strains of trichoderma harzianum s. l. have a great importance because of affecting mankind by being used as biological control agents against plant pathogens. in the present study, we planned to characterize the phylogenetic structure of t. harzianum recovered from different geographical origins and various habitats in some climates of iran (nc, scd, csw). in order to screen 239 collected tric...

Purpose: To quantify the specificity and sensitivity of RTVue-100, Optovue parameters at determining the presence of a relative afferent pupillary defect (RAPD) by coupling their values with the instrument normative data base (NDB) and the presence of a RAPD. To generate the distribution for the focal loss volume (FLV) percentage, by coupling its value to the instrument NDB and presence of a RA...