UNLABELLED PHARMACOLOGICAL RELEVANCE: Presentation of the scratch assay as a convenient and inexpensive in vitro tool to gain first insights in the wound healing potential of plant extracts and natural compounds. AIM OF THE STUDY The present study deals with the optimization of the scratch assay which can be used as an in vitro model for quantification of fibroblast migration to and prolifera...

BACKGROUND Prostate cancer-associated fibroblasts (CAF) can stimulate malignant progression and invasion of prostatic tumour cells via several mechanisms including those active in extracellular matrix; METHODS We isolated CAF from prostate cancer patients of Gleason Score 6-10 and confirmed their cancer-promoting activity using an in vivo tumour reconstitution assay comprised of CAF and BPH1 ...

BACKGROUND ER1626, a novel compound, is a derivate of indeno-isoquinoline ketone. This study was designed to evaluate the biological activity and potential anti-tumor mechanism of ER1626. METHOD MTT assay, scratch assay and flow cytometry were used to determine cell proliferation, cell migration and cell cycle distribution as well as cell apoptosis on human breast cancer MCF-7 cells and endom...

Achillea eriophora D.C. (Asteraceae), an endemic species in Iran, is used extensively in Iranian folk medicine to treat various ailments, including gastrointestinal disorders, skin inflammations and wound healing. The present study was aimed to investigate the effects of the methanolic extract of  A. eriophora leaves on the stimulation of human fibroblast cell proliferation and migration as two ...

Quantifying the ability of a compound to modulate cell migration rate is a crucial part of many studies including those on chemotaxis, wound healing and cancer metastasis. Existing migration assays all have their strengths and weaknesses. The "scratch" assay is the most widely used because it seems appealingly simple and inexpensive. However, the scratch assay has some important limitations, as...

Oral ulcerations often arise as a side effect from chemo- and radiation therapy. In a previous clinical study, Porphyromonas gingivalis was identified as a positive predictor for oral ulcerations after hematopoetic stem cell transplantation, possibly incriminating P. gingivalis in delayed healing of the ulcerations. Therefore, it was tested whether P. gingivalis and its secreted products could ...

This study aimed to investigate the phenolic content, antioxidant activity, cytotoxicity and in vitro wound healing activity of methanolic aqueous extracts Verbascum stachydifolium Boiss & Heldr. var. stachydifolium. Total flavonoid contents were measured using spectrophotometry-based methods. Quantitative analysis selected phenolics was performed by HPLC. The cytotoxic effects on L929 mous...