• End-products of sludge-based AD were used for SCP production the first time. A potential solution to valorize underutilized products generated at WWTPs. Methylomonas dominated in mixed culture. The protein content reached 41% w/w dry weight. Several essential amino acids detected. Single cell (SCP) provides an alternative source partially replace conventional agricultural resources and suppor...

Although sterol carrier protein-2 (SCP-2; also called nonspecific lipid transfer protein) binds fatty acids and fatty acyl-CoAs, its role in fatty acid metabolism is not fully understood. L-cell fibroblasts stably expressing SCP-2 were used to resolve the relationship between SCP-2 intracellular location and fatty acid transacylation in the endoplasmic reticulum. Indirect immunofluorescence dou...

Sterol carrier protein-2 (SCP-2) and SCP-x are ubiquitous proteins found in all mammalian tissues. Although both proteins interact with fatty acids, their relative contributions to the uptake, oxidation, and esterification of straight-chain (palmitic) and branched-chain (phytanic) fatty acids in living cells has not been resolved. Therefore, the effects of each gene product on fatty acid metabo...

Intratumoral genetic heterogeneity may impact disease outcome. Gold standard for dissecting clonal heterogeneity are single-cell analyses. Here, we present an efficient workflow based on an advanced Single-Cell Printer (SCP) device for the study of gene variants in single cancer cells. To allow for precise cell deposition into microwells the SCP was equipped with an automatic dispenser offset c...

Single-cell protein (SCP) from agro-waste material has gained increased attention in the recent past as a relatively cheap and alternative source to meet nutritional demand generated by fast-growing population. Furthermore, bioconversion of these wastes into SCP such value-added products reduce environmental-related issues. In this study, locally available pineapple (Ananas comosus), watermelon...

Sterol carrier protein-2 (SCP-2) plays an important role in cholesterol trafficking and metabolism in mammalian cells. The purpose of this study was to determine whether SCP-2, under oxidative stress conditions, might also traffic hydroperoxides of cholesterol, thereby disseminating their cytotoxic effects. Two inhibitors, SCPI-1 and SCPI-3, known to block cholesterol binding by an insect SCP-2...

The effects sterol carrier protein-2 (SCP-2) expression on L-cell phospholipid levels and fatty acyl composition was assessed using L-cells transfected with the murine cDNA encoding for either the 15 kDa proSCP-2 or 13.2 kDa SCP-2. Expression of these proteins reduced total phospholipid mass (nmol/mg protein) by 24% and reduced the cholesterol to phospholipid ratio 60 and 28%, respectively. In ...

: world population has been continuously raised and therefore due to this fact new source of foods, particularly proteins, are in demand. in recent years, single cell protein has been considered as an accepted substitute for animal and plant proteins. in this study, single cell protein production was studied in a batch submerged culture using aspergillus niger ptcc5012. experimental design was ...

: World population has been continuously raised and therefore due to this fact new source of foods, particularly proteins, are in demand. In recent years, single cell protein has been considered as an accepted substitute for animal and plant proteins. In this study, single cell protein production was studied in a batch submerged culture using Aspergillus niger PTCC5012. Experimental design was ...

Sterol carrier protein (SCP) is a highly abundant, ubiquitous, low molecular weight protein that has a rapid turnover and multifunctional roles in lipid metabolism and transport. It is also known as the fatty acid-binding protein. These and other characteristics led to studies on the possible role of SCP in mediation of steroidogenic responses of the adrenal gland to corticotropin. To quantitat...