Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccine can protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologous re...

AIM This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt. MATERIALS AND METHODS The phenotypic characterization of Salmonella strains was done using standard microbiological, biochemical,...

Most strains of Salmonellae have simple nutritional requirements and grow well, therefore, in simple media consisting of glucose, inorganic nitrogen, and mineral salts (Kauffmann, 1954). There are, however, a number of Salmonellae which are unable to synthesize certain essential vitamins and amino acids and these must be supplied in the culture medium. The need of Salmonella typhosa for tryptop...

hilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to...

Salmonella enterica serovar Typhimurium synthesizes cobalamin (vitamin B12) only during anaerobiosis. Two percent of the S. Typhimurium genome is devoted to the synthesis and uptake of vitamin B12 and to B12-dependent reactions. To understand the requirement for cobalamin synthesis better, we constructed mutants of Salmonella serovars Enteritidis and Pullorum that are double-defective in cobala...

MCCULLOUGH, N. B., AND EISELE, C. W. 1951c Experimental human salmonellosis. III. Pathogenicity of strains of Salmonella newport, Salmonella derby, and Salmonella bareilly obtained from spray-dried whole egg. J. Infectious Diseases, 89, 209-213. MCCULLOUGH, N. B., AND EISELE, C. W. 1951d Experimental human salmonellosis. IV. Pathogenicity of strains of Salmonella pullorum obtained from spray-dr...