Industrially useful polyploid yeasts such as the brewing yeasts do not possess any auxotrophic genetic markers and hence are not easily amenable to plasmid-mediated DNA transformations. In an attempt to obtain genetic markers, a number of useful Saccharomyces sp. strains and some amylolytic Schwanniomyces sp. strains were tested for their susceptibility to the antibiotic Geneticin G418 , a 2-de...

Stationary-phase (SP) batch cultures of Saccharomyces cerevisiae, in which growth has been arrested by carbon-source depletion, are widely applied to study chronological lifespan, quiescence and SP-associated robustness. Based on this type of experiments, typically performed under aerobic conditions, several roles of oxygen in aging have been proposed. However, SP in anaerobic yeast cultures ha...

Surfactant proteins A (SP-A) and D (SP-D) are members of the collectin family of calcium-dependent lectins and are important pulmonary host defense molecules. Human SP-A and SP-D and rat SP-D bind to Aspergillus fumigatus conidia, but the ligand remains unidentified. To identify a fungal ligand for SP-A and/or SP-D, we examined the interactions of the proteins with Saccharomyces cerevisiae. SP-...

Cereal-porridge(‘ogi’) was produced by spontaneous fermentation using maize and sorghum substrates. The microbiological dynamics involved were monitored over a period of 48h fermentation. Bacteria, yeasts moulds isolated. Based on the morphological, cultural biochemical test results, aerobic bacterial isolates identified as Proteus vulgaris, mirabilis, Klebsiella sp, Staphylococcus aureus, Lact...

Production of reducing sugar by hydrolysis of corncob material with Streptomyces sp. cellulase and ethanol fermentation of cellulosic hydrolysate was investigated. Cultures of Streptomyces sp. T3-1 improved reducing sugar yields with the production of CMCase, Avicelase and β-glucosidase activity of 3.8, 3.9 and 3.8 IU/ml, respectively. CMCase, Avicelase, and βglucosidase produced by the Strepto...

Zygomycetes fungi are able to produce ethanol, and their biomass may hold a high market value, making them interesting microorganisms from a biorefinery perspective. In the present study, the inhibitor tolerance of the Zygomycetes fungus Rhizopus sp. was evaluated and compared with a flocculating strain of Saccharomyces cerevisiae. The inhibitors furfural, 5hydroxymethylfurfural [HMF], acetic a...

Candida glabrata is an opportunistic fungal pathogen that can cause severe invasive infections and can evade phagocytic cell clearance. We are interested in understanding the virulence of this fungal pathogen, in particular its oxidative stress response. Here we investigated C. glabrata, Saccharomyces cerevisiae and Candida albicans responses to two different oxidants: menadione and cumene hydr...

    Sucrose hydrolysis was carried Out by Saccharomyces strains. The effective rate of reaction was examined experimentally for 5 different Saccharomyces strains. Two Strains of Schizosaccharomyces and Saccharomyces Carlsbergensis showed the best invertase activity. These two strains showed Michaelien Kinetics in the sucrose concentration up to 0.2 M and inhibitory effect in the concentration b...

Tobacco is a crop of economic importance in tropical countries; it generates significant income small agricultural areas. Within the agrotechnical activities that are carried out on this plant, biofertilization with Autochthonous Microorganisms (AM) represents viable option for production low inputs. Therefore, objective research was to determine biofertilizer effect AM black tobacco cv. Havana...