One hundred sixty-one strains of adherent bacteria were isolated under anaerobic conditions from four sites on the rumen epithelial surface of sheep fed hay or a hay-grain ration. Before isolation of bacteria, rumen tissue was washed six times in an anaerobic dilution solution, and viable bacteria suspended in the washings were counted. Calculation indicated that unattached bacteria would have ...

The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found i...

That microbial fermentation in the rumen can progress without added amino acids, peptides or proteins was elegantly demonstrated by Virtanen (1966) who maintained lactating dairy cows for prolonged periods on purified diets with urea as the only N source. However, there have also been numerous studies which have demonstrated benefits of feeding pre-formed amino acids, either as free amino acids...

From the simultaneous accumulation of hydrogenation intermediates and the disappearance of Isotricha prostoma after algae supplementation, we suggested a role of this ciliate and/or its associated bacteria in rumen biohydrogenation of unsaturated fatty acids. The experiments described here evaluated the role of I. prostoma and/or its associated endogenous and exogenous bacteria in rumen biohydr...

Increasing awareness of hazards associated with the use of antibiotic and chemical feed additives has accelerated investigations into plants and their extracts as feed additives. The present review mainly discusses two classes of plant secondary compounds in this context, i.e. essential oils and saponins. The broader potential of plants and their extracts is illustrated by the progress of an EC...

Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganis...

introduction: sugarcane (saccharum officinarum) is widely cultivated in south west of iran for production of sugar. after harvest, a lot of sugarcane tops remain on the farm that can be used to feed ruminants. the total digestible nutrients (tdn) and crude fiber of sugarcane top is about 72% and 39%, respectively. the digestibility of dry matter (54%) and crude protein (39%) of sugarcane top is...

this experiment was conducted to determine the effect of procceesing of sugarcane pith with high pressure steam (210-220c◦, at 19 bar, over 3 min, on 70 % moisture) on fermentative activity and gas production parameters and degradation by pure microorganism of rumen. the microbial different groups of rumen were separated by physical and chemical methods and using antibiotics. these microbial gr...

Aims The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh's cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzym...

Introduction. The toxic nonprotein amino acid mimosine present in Leucaena leucocephala is degraded by rumen bacteria to produce another toxic compound, 3-hydroxy-4(lH)-pyridone (3,4-dihydroxypyridine, or 3,4-DHP). In some, but not all, geographic areas ruminants are colonized by bacteria that degrade 3,4-DHP, and actions of these microbes protect their host animals from leucaena toxicosis (Jon...