In this study, protein domains with cellulase activity in goat rumen microbes were investigated using metagenomic and bioinformatic analyses. After the complete genome of goat rumen microbes was obtained using a shotgun sequencing method, 217,892,109 pair reads were filtered, including only those with 70% identity, 100-bp matches, and thresholds below E(-10) using METAIDBA. These filtered conti...

Pastoral agriculture in New Zealand has a long history of improving the efficiency with which fresh pasture is converted into meat, wool and dairy products. New Zealanders live in a temperate climate where rainfall and pasture growth are the main driving forces in low-input pastoral systems. The process whereby protein in fresh pasture is broken down in the rumen and reassimilated into microbia...

BACKGROUND Lignocellulosic biomass is the most abundant resource on earth. Lignocellulose is mainly composed of cellulose, hemicelluloses, and lignin. The special construction of three kinds of constituents led to the prevention of effective degradation. The goal of this work was to investigate the great potentials of bovine rumen for novel cellulolytic bacterial isolation, which may be used fo...

Considerable difficulty and uncertainty can be associated with sampling through cannulas from the reticulo-rumen of the sheep. The contents are by no means homogeneous, and for some purposes relatively large samples have to be withdrawn from various places within the organ and mixed in an external container before a subsample is taken for analysis and the unused portion returned to the rumen. I...

Effects of amount of feed intake on in vivo remen propionate production and the reproducibility of measurements of propionate production rates were investigated in five dairy steers weighing 142 to 228 kg. Each steer received 275 g of an 80%-grain diet every 2 hours, and three of the five steers later received 500 g/2 hours. Rumen propionate pool sizes and production rates were determined at bo...

The Effects of using Acid-Consuming Bacteria and Different Buffers with High Concentration Diets on In Vitro Digestion Fermentation Activity Anaerobic Fungi Fattening Lambs Rumen

Materials and Methods Dietary supplementation of probiotic yeast ( ) was noted to improve the animal performance by improving the digestibility of nutrients and rumen bacterial number [1]. Addition of yeast culture, as a probiotic, in animal feeds improves fibre digestion [2], the cellulolytic activity of rumen microbes [3], and reduces the lactate accumulation and oxygen concentration in rumen...

Received: Revised: Accepted: September 18, 2014 September 27, 2014 October 15, 2014 In this study, the effect of fermentative disorders such as acid and alkaline indigestion was investigated in buffaloes. Six buffaloes each suffering from acid and alkaline indigestion was chosen and various physiological parameters were compared with six healthy buffaloes Clinical examination was carried out to...

The objective of this paper was to report the isolation of two fluoroacetate degrading bacteria from the rumen of goats. The animals were adult goats, males, crossbred, with rumen fistula, fed with hay, and native pasture. The rumen fluid was obtained through the rumen fistula and immediately was inoculated 100 μL in mineral medium added with 20 mmol L(-1) sodium fluoroacetate (SF), incubated a...

The objective of this study was to investigate the effect of NDF content in highly digestible grass/clover silage on particle size reduction, ruminal stratification, and selective retention in dairy heifers. The reduction in particle size from feed to feces was evaluated and related to feed intake, chewing activity, and apparent digestibility. Four grass/clover harvests (Mixtures of Lolium pere...