The COVID-19 pandemic has underscored the shortcomings in deployment of state-of-the-art diagnostics platforms. Although several polymerase chain reaction (PCR)-based techniques have been rapidly developed to meet growing testing needs, such often need samples collected through a swab, use RNA extraction kits, and expensive thermocyclers order successfully perform test. Isothermal amplification...

A rapid, cost-effective diagnostic test for the detection of acute HIV-1 infection is highly desired. Isothermal amplification techniques, such as reverse-transcription loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for the development of a rapid nucleic acid amplification test (NAAT) because they are quick, easy to perform and do not require complex, d...

The development and validation of a one-step, real-time, and quantitative dengue virus serotype-specific reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the 3' noncoding region for the rapid detection and differentiation of dengue virus serotypes are reported. The RT-LAMP assay is very simple and rapid, wherein the amplification can be obtained in 30 min u...

The SARS-CoV-2 pandemic has forced all countries worldwide to rapidly develop and implement widespread testing control manage the Coronavirus Disease 2019 (COVID-19). reverse-transcription (RT)-qPCR is gold standard molecular diagnostic method for COVID-19, mostly in automated platforms. These systems are accurate effective, but also costly, time-consuming, high-technological, infrastructure-de...

The standardization and validation of a one-step, single-tube accelerated quantitative reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay is reported for rapid and real-time detection of Japanese encephalitis virus (JEV). The RT-LAMP assay reported in this study is very simple and rapid; the amplification can be obtained in 30 min under isothermal conditions at 63 degr...

The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, that arose in 2019 underscored significance of testing and diagnostics order to combat rapid spread disease. most common method for SARS-CoV-2 RNA detection has been RT-qPCR method, which is insufficient terms meeting immense demand due its relatively high time-consumption. Under these circumstances, urgent need a similarly sens...

Chrysanthemum chlorotic mottle viroid (CChMVd) is a serious pathogen infecting chrysanthemum worldwide. To improve and enhance the detection procedure, colorimetric loop-mediated isothermal amplifi cation (LAMP) technique was developed. Six LAMP primers were newly designed tested optimal conditions using recombinant plasmid of CChMVd as DNA template. The for incubation at 65°C 60 min. At these ...

Abstract Background Rapid spread of SARS-CoV-2 has led to a global pandemic, resulting in the need for rapid assays allow diagnosis and prevention transmission. Reverse transcription-polymerase chain reaction (RT-PCR) provides gold standard assay RNA, but instrument costs are high supply chains potentially fragile, motivating interest additional methods. transcription loop-mediated isothermal a...