New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from genomic DNA. RSD-PCR strategy is based on these principles: (i) restriction sites disperse throughout...

Immunoglobulin G4-related sclerosing disease (IgG4-RSD) is a fibroinflammatory condition that has the potential to affect nearly every organ system. Classic histological findings include storiform fibrosis and lymphoplasmacytic infiltrates of immunoglobulin G4 (IgG4)-positive plasma cells. The clinical features of IgG4-RSD may be an under-recognized disease process that can mimic other autoimmu...

A radiation sterilization dose (RSD) of 25 kGy is deleterious to bone allografts. This study aimed to establish a lower RSD for bone allografts using method 1 of International Standard Organisation 11137.2:2006. This provides a database to select an RSD corresponding to an allograft's bioburden, given that the bioburden's gamma resistance is equal to or less than the standard. This can be verif...

[Purpose] To evaluate the relationships between residual strength deficits (RSD) of the upper limb muscles and the performance in bimanual activities and to determine which muscular group would best explain the performance in bimanual activities of chronic stroke individuals. [Subjects and Methods] Strength measures of handgrip, wrist extensor, elbow flexor/extensor, and shoulder flexor muscles...

Surgical renal sympathetic denervation (RSD) has been used to control resistant hypertension.1 With the development of catheter based RSD, this old technique has been resuscitated for the treatment of hypertension. While initial clinical studies showed effective hypertension control,2 a recent sham-controlled randomized clinical trial failed to confirm its antihypertensive efficacy.3 These nega...

Background: There is inconsistent information about the role of psychological factors in the incidence of reflex sympathetic dystrophy (RSD) in patients with distal radius fractures. The present study was thus undertaken to evaluate the relationship between some of these factors and the occurrence of reflex sympathetic dystrophy in patients with distal radius fracture. Methods: One hundred and ...

The Escherichia coli Rsd protein forms 1 : 1 complexes with sigma(70) protein, which is the major factor in determining promoter recognition by RNA polymerase. Here we describe measurements of the levels of Rsd, RNA polymerase, sigma(70) and the alternative sigma(38) factor. Rsd levels are sufficient to sequester a significant proportion of sigma(70) and immunoaffinity pull-down experiments sho...

BACKGROUND The purpose of this study was to investigate whether transcatheter renal sympathetic denervation (RSD) interfere with the development of left ventricular (LV) mechanical dyssynchrony during the progression of heart failure (HF). METHODS Nineteen beagles were randomly divided into sham-operated group (six dogs), control group (seven dogs), and RSD group (six dogs). Sham-operated gro...

The environmental disaster following flooding by red sludge in the Ajka region in Hungary poses a seriouspublic health threat with particular concern regarding the potentially adverse respiratory effects of theinhalation of red sludge dust (RSD). The respiratory consequences of the inhalation of RSD obtained fromfield samples were investigated in rats. Rats were either exposed to RS...

The history of medicine provides perspective invisible from PubMed. Past theories and practices seem risible until one considers which moderns medical ''facts " will amuse future generations. Neurological disorders have been particularly difficult to resolve since anatomy and function are so complex. Primitive societies blamed the gods for seizures and strokes. Disease causality was eventually ...