Five wild plant species belonging to different families (Chenopodium album, Plantago major, Elytrigia elongata, Filipendula ulmaria and Nigella sativa) widely spread in Russian Federation and the former USSR were evaluated for their ability to inhibit growth of two important human food-borne pathogens (Escherichia coli O157:H7 and Listeria monocytogenes strain EGD-e) and eight plant pathogens (...

A new RP-HPLC method was developed for selective and simultaneous determination of of Desogestrel and Ethinyl Estradiol Tablets. The chromatographic separation was achieved on a Zorbax SB C-18, 4.6 x 250mm, and 5μ column. The gradient LC method employs solutions A and B as mobile phase. The solution A milliQ water and acetonitrile in the ratio of 90:10 and degas and solution B contains a mixtur...

Cereal Chern. 65(3):215-222 Zein, the major alcohol-soluble protein of corn endosperm, was extracted with 55% 2-propanol. It was then fractionated by preparative reversed-phase (CIs) high-performance liquid chromatography (RPH PLC) with an acetonitrile-triOuoroacetic acid gradient. From 15 to 17 peaks resulted for native or reduced and alkylated (R-A) zeins. Sodium dodecyl sulfate-polyacrylamid...

Abstract: Aim: A new stability indicating RP-HPLC based methods were developed to identify the possible degradation products of Ivermectin in isolated rat hepatocytes and different pH media using a C18 RP column. Complete validation, including linearity, accuracy, recovery, precision, robustness, stability, peak purity, was performed. Materials Methods: HPLC system UltiMateDionex 3000 DAD with ...

Forty-seven saturated synthetic diacyl PA, PC, PE, PG, and PS and five unsaturated diacyl phospholipids (PL) underwent normaland reversed-phase (RP) HPLC with isocratic isopropanol/hexane/water (5∶4∶1) and methanol/chloroform/acetonitrile/water (79.5∶9∶8∶3.5) mobile phases, respectively. For normal-phase HPLC, capacity factors (k′ i ) decrease with chain length (n) of the two identical PL FA re...

Snake venoms comprise a highly complex mixture of proteins, and there is also high interspecific intraspecific variability in their composition, even the same region. Our aim was to compare composition Bothrocophias myersi, Crotalus durissus Bothrops asper, snakes from Andean region Colombia by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). The were given research group under a...

An rp-hplc method for the simultaneous determination of Ramipril (RP) and Amlodipine (AL) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01-0.25 mg/mL (r2=0.9998) for RP and 0.014-0.36 mg/mL (r2=0.9997) for AL. The limits of detection (LOD) were 0.06 μg/mL and 0.02 μg/mL for RP and AL, and the limits ...

A simple, sensitive and precise RP-HPLC method was developed for the determination of dutasteride in tablet dosage form. The RP-HPLC separation was achieved on phenomenex C(18) column (250 mm, id 4.6 mm, 5 mum) using mobile phase methanol:water (90:10 v/v) at a flow rate of 1 ml/min at an ambient temperature. Quantification was achieved with photodiode array detection at 235 nm over the concent...