tissue culture methods provide tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, in vitro propagation of diaphanoptera khorasanica rech.f., a rare and threatened plant species with limited distribution range and population was investigated. this species has a potential as an ornamental plant. single node explants wer...

In this research, a factorial 4×3×4 with 4 replications in Completely Randomized design was used to study the effects of different auxin treatments (4 levels), pH(2 levels) and cultivars (3 levels) on regeneration and rooting of in vitro shoot tips excised from shoots cultured on media supplemented with 1.5-2 mg/l BA. The results showed both auxins (NAA and IBA) produced callus in the bases of ...

With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the...

Rubber tree (Hevea brasiliensis) self-rooting juvenile clones (JCs) are promising planting materials for rubber production. In a comparative trial between self-rooting JCs and donor clones (DCs), self-rooting JCs exhibited better performance in rubber yield. To study the molecular mechanism associated with higher rubber yield in self-rooting JCs, we sequenced and comparatively analyzed the late...

Asexual propagation of pomegranate is highly confined to hardwood cutting but there a grate scope air layering method exploit its potential. To understand this, an investigation was carried out at the student instructional field, department Horticulture, School Agricultural sciences, G. H. Raisoni University Saikheda, Saunsar, Distt- Chhindwara (M.P.) during rainy season year 2021-22. The treat...

in order to investigate the in vitro micropropagation hibiscus rosa-sinensis three tests in a completely randomized design with 10 replications were performed in tissue culture laboratory shahid chamran university in 2014.the first experiment showed the perfect media.second tests best consentration bap for multiplication and third tests showed the best type and concentration rooting hormone. co...

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and M...

The establishment of a vegetative cover to enhance evapotranspiration and control runoff and drainage was examined as a method for stabilizing a landfill containing coal combustion waste. Suitable plant species and pretreatment techniques in the form of amendments, tilling, and chemical stabilization were evaluated. A randomized plot design consisting of three subsurface treatments (blocks) and...

Lycium chilense, a deciduous perennial shrub, is one of the endangered native species of Patagonia due to sheep overgrazing. Chances of recolonization by seeds are scarce due to the limited density of propagules in the soil and very specific requirements for germination. The objective was to develop an in vitro propagation protocol that would help to perform reestablishment of this species in d...