in this study, the effects of dietary egg lecithin on digestive enzymes and body biochemical composition of juveniles mesopotamichthys sharpeyi was evaluated. four experimental diets including control diet (with 0% egg lecithin) and three diets containing 2%, 4% and 6% egg lecithin were used. at the end of the experiment, digestive enzymes activity (lipase, amylase and alkaline phosphatase) and...

the main purpose of this project is investigation of the organophosphorus hydrolase (oph) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the oph enzyme stability in formulated creams. oph enzyme was extracted and purified from strain flavobacterium. the w/o and o/w creams were prepared using different formulations. in order to achieve an emulsion with m...

conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...

Objective: Acute chest pain is an important and frequently occurring symptom in patients. Chest pain is often a sign of ischemic heart disease. Chest pain due to suspected Acute Coronary Syndrome (ACS) is responsible for a large and ijncreasing number of hospital attendances and admissions. Current practice for suspected ACS involves troponin testing 10–12 hours after symptom o...

Even purified enzyme preparations are often heterogeneous. For example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme.  For this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the characteristics of the species present.  Based...

Background & Aims: Chronic pulmonary infection in patients with cystic fibrosis is predominantly due to infection by mucoid strains of Pseudomonas aeruginosa. Mucoid P. aeruginosa is due to the production of exopolysaccharide called also alginate. Alginate in addition to interference with the clearance of lung has antiphagocytic property. Optimal killing activity of P. aeruginosa requires opson...

The laccase enzyme is the largest group of Oxidoreductase enzymes and is capable of oxidizing a wide range of organic substrates to water along with molecular oxygen resuscitation. ZnO nanoparticles are known for their specific properties such as chemical stability, high electrochemical coupling rates, and wide range of absorption of radiation as multifunctional compounds. In this study, ZnO po...

This work addresses the binding, cleavage and dissociation rates for the substrate and products of a synthetic RNaseA mimic that was combinatorially selected using chemically modified nucleoside triphosphates. This trans-cleaving DNAzyme, 9(25)-11t, catalyzes sequence-specific ribophosphodiester hydrolysis in the total absence of a divalent metal cation, and in low ionic strength at pH 7.5 and ...