Protein motions play important roles in numerous biological processes such as enzyme catalysis, muscle contractions, antigen-antibody interactions, gene regulation and virus assembly. Knowledge of protein flexibility is also important in rational drug design, protein docking and protein engineering. However, the experimental measurement of protein motions is often difficult, requiring sophistic...

Microvascular thrombi underlie many of the clinical manifestations of Rocky Mountain spotted fever (RMSF), a disease characterized by Rickettsia rickettsii infection of vascular endothelial cells. Studies were designed to determine whether R rickettsii-infection of cultured human umbilical vein endothelial cells results in tissue factor (TF) induction, a process that could directly activate coa...

R ocky Mountain spotted fever (RMSF), a classic metazoonosis that involves both vertebrate and non-vertebrate reservoir hosts, is a seasonal disease of dogs and humans in the Americas. The clinical illness was first described among Native Americans, soldiers, and settlers in the Bitterroot River and Snake River valleys of Montana and Idaho during the late 1890s, but remained unrecognized in dog...

Most emerging infectious diseases today, such as West Nile virus and sudden acute respiratory distress syndrome (SARS), arise from zoonotic reservoirs and many are transmitted by arthropod vectors. Ticks are among the most competent and versatile arthropod vectors of infectious diseases because ticks of all ages and both sexes remain infectious for generations without having to reacquire infect...

Microvascular thrombi underlie many of the clinical manifestations of Rocky Mountain spotted fever (RMSF), a disease characterized by Rickettsia rickettsii infection of vascular endothelial cells. Studies were designed to determine whether R rickettsii-infection of cultured human umbilical vein endothelial cells results in tissue factor (TF) induction, a process that could directly activate coa...

Glucagon-like peptide-1 (GLP-1) is a promising target for diabetes mellitus (DM) therapy and reduces the occurrence of diabetes due to obesity. However, GLP-1 will be hydrolyzed soon by the enzyme dipeptidyl peptidase-4 (DPP-4). We tried to design small molecular drugs for GLP-1 receptor agonist from the world's largest traditional Chinese medicine (TCM) Database@Taiwan. According to docking re...

Kanker payudara menjadi salah satu jenis kanker dengan penderita terbanyak baik di dunia maupun Indonesia, Reseptor Estrogen Alfa (ER-α) target utama karena dapat mengatur transkripsi gen dan jalur persinyalan interseluler. Penelitian ini bertujuan untuk menganalisis afinitas kestabilan ikatan kompleks ligan senyawa turunan furanokumarin reseptor estrogen alfa. Metode yang digunakan secara in s...

The clinical manifestations of Rocky Mountain spotted fever (RMSF) result from Rickettsia rickettsii (R rickettsii) infection of endothelial cells and are mediated by pathologic changes localized to the vessel, including in situ thrombosis and tissue ischemia. This study uses in vitro infection of cultured human umbilical vein endothelial cells with R rickettsii to test the hypothesis that such...