We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding cholera toxin. As previously reported, we characterized two populations among toxig...

Clostridium difficile colonization in pig intestine has been a public health concern. We analyzed C. difficile prevalence among piglets in Japan to clarify their origin and extent of the associated risk by using molecular and microbiological methods for both swine and human clinical isolates and foreign isolates. C. difficile was isolated from 120 neonatal piglet fecal samples. Toxin gene profi...

In this article, we analyzed the origin of wild polyploid oats (Avena L., Poaceae) using region 18S rDNA (partially)–ITS1–5.8S obtained via NGS. There are six tetraploid (2n = 28) and four hexaploid 42) species differing by specific genome combinations: A. barbata, vaviloviana (AB), agadiriana (AB or BB), magna, murphyi, insularis (AC CD), ludoviciana, sterilis, fatua, occidentalis (ACD). We co...

Until the emergence of Vibrio cholerae O139 in 1992, toxigenic strains of V. cholerae O1 were considered to be the only causative agents of epidemic and pandemic cholera (3). Molecular studies using pulsed-field gel electrophoresis (4), ribotyping (3), enterobacterial repetitive intergenic consensus sequence (ERIC)-PCR (1), and restriction fragment length polymorphism analysis of the CTX geneti...

There are limited national epidemiological data for community-associated (CA)-Clostridium difficile infections (CDIs). Between March 2011 and March 2013, laboratories in England submitted to the Clostridium difficile Ribotyping Network (CDRN) up to 10 diarrhoeal faecal samples from successive patients with CA-CDI, defined here as C. difficile toxin-positive diarrhoea commencing outside hospital...

We read with great interest the study by Walker et al examining the impact of Clostridium difficile infection (CDI) on 14-day attributable mortality [2]. The authors drew conclusions about C. difficile genotype-specific mortality, noting higher attributable mortality for clades 2 (ribotype 027) and 5 (ribotype 078) [2]. Although the statistical methods appear to be adequate, the presentation of...

Clostridium difficile is one of the main etiological agents causing antibiotic-associated diarrhea. This study investigated the genetic diversity of 70 toxigenic C. difficile isolates from two Korean hospitals by employing toxinotyping, ribotyping, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Toxin gene amplification resulted in 68 A⁺B⁺ and two A-B+ isolates. ...

A study was conducted with the RiboPrinter, an automated ribotyping system, to evaluate its ability to identify and characterize isolates of Salmonella from broiler operations. Isolates of Salmonella obtained from a local broiler company were serotyped by a reference laboratory and ribotyped using the RiboPrinter. The RiboPrinter generated ribotype patterns by probing EcoRI digests of Salmonell...

1. Abstract 2. Introduction 3. Phenotypic techniques 3.1. Serotype analysis 3.2. Biotype and phage type analysis 3.3. Antibiotic susceptibility patterns 4. Genotypic techniques 4.1. Plasmid analysis 4.2. Chromosomal restriction fragment analysis and pulsed-field gel electrophoresis (PFGE) 4.3. Chromosomal restriction-hybridization techniques 4.3.1. Ribotype analysis 4.3.2. IS200 4.4. Locus-spec...

Clostridium difficile is an emerging threat in hospital environments. To analyse possible transmission and to distinguish between relapse and reinfection a collection of C. difficile isolates, sampled from 162 consecutive episodes of C. difficile infection, were PCR ribotyped. Two ribotypes (001 and 012) were prone to cause nosocomial acquisition. Moreover, ribotype 001 had a tendency to cause ...