An affinity chromatographic procedure, using ribosomal or tRNA covalently coupled to agarose through an adipic acid dihydrazide spacer, has been developed to isolate and identify the Escherichia coli ribosomal proteins which specifically bind to these RNA molecules. Transfer RNA, 5-S, 16-S and 23-S ribosomal RNA were readily immobilized on agarose-dihydrazide: the maximum amount coupled decreas...

The Krr1 protein of Saccharomyces cerevisiae is involved in processing of pre-rRNA and assembly of pre-ribosomal 40S subunits. To further investigate the function of Krr1p we constructed a conditional cold sensitive mutant krr1-21, and isolated seven genes from Schizosaccharomyces pombe whose products suppressed the cold sensitive phenotype of krr1-21 cells. Among the multicopy suppressors we f...

Growing cells coordinate protein translation with metabolic rates. Central to this coordination is ribosome production. Ribosomes drive cell growth, but translation of ribosomal proteins competes with production of non-ribosomal proteins. Theory shows that cell growth is maximized when all expressed ribosomes are constantly translating. To examine whether budding yeast function at this limit of...

Rat liver ribosomes have been acetylated both in vivo and in vitro. The in vivo acetylation of ribosomes occurred within 5 min of the administration of t3H]acetate, and 70 to 80% of the incorporated radioactivity remained in the ribosomal proteins after removal of RNA with urea-LiCl. Following treatment of the acetylated ribosomai proteins with ethanol-ether, RNase, and 0.5 M KCl, to remove pos...

Ribosomes are structurally complex cell organelles consisting of ribosomal RNA and a number of different protein molecules. The complexity of these particles raises two major questions: What is the relationship between structure and function of the ribosome, and what is the mechanism of assembly of the ribosome? It is obvious that the study of these two questions would be greatly aided if a met...