Germination and seedling development are crucial phases in a plant’s life cycle with economical agronomical implications. The RNA quality seeds is linked to seed viability, being an important agronomic trait since this leads loss germination efficiency. In addition, can be phosphorous reservoir seeds, affecting the efficiency of mobilisation nutrients towards seedlings. However, knowledge about...

Rubella virus ribonucleoprotein was accessible to pancreatic ribonuclease, Pronase, and certain polyanions. Most of the ribonucleic acid (RNA) label was made acid-soluble by ribonuclease, whereas Pronase and the polyanions liberated 40S RNA from the ribonucleoprotein.

1. Extracellular ribonuclease is produced linearly for at least 3hr. by washed post-logarithmic-phase cells of Bacillus subtilis suspended in a medium containing maltose (1%) and casein hydrolysate (0.5%). 2. Low concentrations of actinomycin D (less than 2mug./ml.) stimulate ribonuclease formation, the maximum effect being observed with a concentration of 1mug./ml. Concentrations greater than ...

The incorporation of the cytokinin N(6)-benzyladenine into tobacco (Nicotiana tabacum) callus tRNA and rRNA preparations isolated from tissue grown on medium containing either N(6)-benzyladenine-8-(14)C or N(6)-benzyladenine-8-(14)C: benzene-(3)H(G) has been examined. N(6)-benzyladenine was incorporated into both the tRNA and rRNA preparations as the intact base. Over 90% of the radioactive N(6...

Partial renaturation of urea-denatured bovine pancreatic ribonuclease by polyanions such as phosphate and polyphosphates, as well as by nucleotides, has been observed by Sela, Anfinsen, and Harrington (1). The finding that the velocities of denaturation and renaturation of ribonuclease may be separately and easily measured (2) prompted us to reinvestigate by kinetic methods the manner by which ...

Bovine seminal ribonuclease (BS-RNase), a dimeric homolog of bovine pancreatic ribonuclease A (RNase A), is toxic to mammalian cells. In contrast to dimeric BS-RNase, a monomeric BS-RNase and RNase A are not cytotoxic and are bound tightly by cytosolic ribonuclease inhibitor. To elucidate the mechanism of ribonuclease cytotoxicity, we constructed a series of hybrid and semisynthetic enzymes and...

Select members of the bovine pancreatic ribonuclease A (RNase A) superfamily are potent cytotoxins. These cytotoxic ribonucleases enter the cytosol, where they degrade cellular RNA and cause cell death. Ribonuclease inhibitor (RI), a cytosolic protein, binds to members of the RNase A superfamily with inhibition constants that span 10 orders of magnitude. Here, we show that the affinity of a rib...

The rate of formation of ribonuclease-S from ribonuclease A by the action of subtilisin was measured at pH 5.5 in the presence and absence of the strongly bound ribonuclease inhibitor, 2’-cytidylate. Presence of the inhibitor in the active center significantly reduced the rate of formation of ribonuclease-S. Since bond 20-21 (and, to a lesser extent, 21-22), the cleavage of which is responsible...