BACKGROUND We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the enzyme activity and amount of enzyme produced were not adequate for industrial application. Our go...

Structured lipids (SLs) containing palmitic, oleic, stearic, and linoleic acids, resembling human milk fat (HMF), were synthesized by enzymatic acidolysis reactions between tripalmitin, hazelnut oil fatty acids, and stearic acid. Commercially immobilized sn-1,3-specific lipase, Lipozyme RM IM, obtained from Rhizomucor miehei was used as the biocatalyst for the enzymatic acidolysis reactions. Th...

The biochemical activity of enzymes, such as lipases, is often associated with structural changes in the enzyme resulting in selective and stereospecific reactions with the substrate. To investigate the effect of a substrate and its chain length on the dynamics of the enzyme, we have performed molecular dynamics simulations of the native Rhizomucor miehei lipase (Rml) and lipase-dialkylphosopha...

Different lipase enzymes have been tested in order to perform regioselective acetylations on the eudesmane tetrol from vulgarin. High yields (95%) of 1,12-diacetoxy derivative (4) were achieved in 1 h with Candida antarctica lipase (CAL). However, only the 12-acetyl derivative (6) was obtained in similar yield with Mucor miehei (MML) or Candida cylindracea (CCL) lipases. The enzymatic protectio...

We have used continuum electrostatic methods to investigate the role of electrostatic interactions in the structure, function, and pH-dependent stability of the fungal Rhizomucor miehei lipase (RmL) family. We identify a functionally important electrostatic network which includes residues S144, D203, H257, Y260, H143, Y28, R80, and D91 (residue numbering is from RmL). This network consists of r...

The synthesis of structured lipids with nutraceutical applications, such as medium-long-medium (MLM) triacylglycerols, via modification oils and fats represents a challenge for the food industry. This study aimed to synthesize MLM-type dietary triacylglycerols by enzymatic acidolysis cottonseed oil capric acid (C10) catalyzed Lipozyme RM IM (lipase from Rhizomucor miehei) in fluidized bed react...

The present work reports on the use of a hybrid blend biopolymers as matrix for lipase immobilization. (Hydroxypropyl)methyl cellulose (HPMC) and Chitosan (CS) were combined in order to formulate film which Mucor miehei was immobilized. biocatalyst studied upon model reaction propyl laurate synthesis. system examined terms its capability provide an appropriate environment where will maintain ac...

Abstract A new approach was used for the immobilization of Thermomyces lanuginosus lipase (TLL), Candida antarctica B (CALB), and Rhizomucor miehei (RML) on amine-functionalized magnetic nanoparticles (Fe 3 O 4 @SiO 2 -NH ) via a multi-component reaction route (using cyclohexyl isocyanide). The method offered single-step very fast process covalent attachment lipases under extremely mild conditi...