We tested the pathogenic role of O2-) radicals in excitotoxic injury. Inactivation of the TCA cycle enzyme, aconitase, was used as a marker of intracellular O2- levels, and a porphyrin SOD mimetic was used to scavenge O2-. The selective, reversible, and SOD-sensitive inactivation of aconitase by known O2- generators was used to validate aconitase activity as a marker of O2- generation. Treatmen...

This work focuses, for the first time, on mechanistic modelling of wastewater disinfection by solar photo-Fenton process using controlled simulated radiation. At circumneutral pH, iron precipitates forming hydroxides and significantly affecting optical properties water. In this regard, radiation transfer in reactor has been taken into account. The proposed model considers that inactivation bact...

Inducible and reversible silencing of selected neurons in vivo is critical to understanding the structure and dynamics of brain circuits. We have developed Molecules for Inactivation of Synaptic Transmission (MISTs) that can be genetically targeted to allow the reversible inactivation of neurotransmitter release. MISTs consist of modified presynaptic proteins that interfere with the synaptic ve...

Recent’ work on the reactivation and reconstitution of several denatured enzymes has provided increasing understanding of the process of reversible protein denaturation. Studies on ribonuclease by Anfinsen et al. (1) and White (2) have convincingly demonstrated that under favorable conditions a complet,ely denatured protein can resume the active three-dimensional configuration of the native mol...

Several known inhibitors of mammalian ribonucleotide reductase were studied for their interactions with herpes simplex virus type 1 (HSV-1) ribonucleotide reductase. MAIQ (4-methyl-5-amino-l-formylisoquinoline thiosemicarbazone) produced apparent inactivation of HSV-1 ribonucleotide reductase. Only catalytically cycling, not resting, enzyme could be inactivated. Double reciprocal replots of the...

Soluble 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) from rat liver microsomes has been shown to be inactivated reversibly by cold temperature whereas microsomal enzyme did not show any cold sensitivity. At 0” the soluble enzyme lost 80% of its activity within 30 min and warming at 37” for 20 min restored all the lost activity. Active enzyme preparations did ...

Studies of the chemical properties of the enzyme, catecholase, have shown that this polyphenoloxidase consists of a copper-protein complex (l-3). Indications have also been obtained that the enzymic activity of catecholase is associated with the copper part of the molecule, since it has been found that the addition of certain reagents known to react with cupric ions will inhibit the enzymic act...

Inactivation permits direct assessment of the functional contribution of a given brain area to behavior. Previous inactivation studies of the frontal eye field (FEF) have either used large permanent ablations or reversible pharmacological techniques that only inactivate a small volume of tissue. Here we evaluated the impact of large, yet reversible, FEF inactivation on visually guided, delayed,...

we introduce the notion ofstrongly $alpha$-reversible rings which is a strong version of$alpha$-reversible rings, and investigate its properties. we firstgive an example to show that strongly reversible rings need not bestrongly $alpha$-reversible. we next argue about the strong$alpha$-reversibility of some kinds of extensions. a number ofproperties of this version are established. it is shown ...