Antibody-free approaches for quantitative LC-MS/MS-based protein bioanalysis are reviewed and critically evaluated, and compared with the more widely used immunoaffinity-based approaches. Antibody-free workflows will be divided into four groups and discussed in the following order: direct analysis of signature peptides after proteolytic digestion; enrichment of target proteins and signature pep...

The pore-forming microcin E492 was purified by solid-phase extraction and reversed-phase high-pressure liquid chromatography. Its molecular mass was 7,886 Da. The entire 84-amino-acid sequence was determined. There is no postranslational modification in the secreted microcin, and the sequence has homologies with the sequence of the microcin colicin V.

A method combining solid-phase extraction and reversed-phase high-performance liquid chromatography is described for the isolation of two key metabolites in the limonoid biosynthetic pathway critical to citrus quality. Potassium salts of limonoate A-ring lactone and nomilinoate A-ring lactone were isolated from young Chandler pummelo seedlings and characterized on the basis of proton and carbon...

acetonitrile used as a solvent in manufactures and affects to central nervous system from inhalation exposure. the aim of this study was to develop a micro-solid phase extraction method for  the determination of acetonitrile in the air matrix. the sampling was performed with a small diameter fused silica fiber coated with a thin film of stationary phase and was subsequently desorbed and analyze...

Proteins can be extracted from an aqueous phase into reversed micelles. This transfer is governed by electrostatic interactions, for protein and surfactant have to bear opposite charges. The presence of high salt concentrations diminishes the attractive interactions and can lead to expulsion of the solubilized proteins. This is used to recover extracted proteins. Larger proteins require a large...

Affinity-based reversed micellar extraction and separation (ARMES) is an effective method for purifying both low and high molecular weight glycoproteins via liquid-liquid extraction. A range of extraction conditions were examined to gain insight into the mechanism of ARMES. Concanavalin A (Con A) was used as the model affinity ligand to bind soybean peroxidase (SBP) and beta-galactosidase as mo...

A reversed-phase salt assisted liquid-liquid extraction (RP-SALLE) method was developed for the preconcentration and determination of trace crocin in herbal medicines by high-performance liquid chromatography (HPLC). In developed method, the roles of the aqueous and organic phases are inverted so that the analyte is extracted from the organic into the aqueous phase after salt addition and phase...