canine distemper virus (cdv) is the cause of canine distemper (cd) which is a severe and highly contagious disease in dogs. in the present study, a duplex reverse transcription polymerase chain reaction (rt-pcr) method was developed for the detection and differentiation of wild-type and vaccine strains of cdv. four primers were designed to detect and discriminate the two viruses by generating 6...

Enteroviruses are the causative agents of a number of diseases in humans. Group B coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. Genomic data of enteroviruses has allowed developing new molecular approaches such as Nucleic Acid Sequence Based Amplification (NASBA) for detection of such viruses. In this study, coxsackievirus B3 (CVB3) was de...

Objective: To establish the accuracy of computed tomography(CT) chest in diagnosis COVID-19 pneumonia by taking reverse transcriptase-polymerase chain reaction(RT-PCR) as a reference standard and to analyze discordant CT RT-PCR results.Materials Methods: A retrospective cross-sectional study patients presented tertiary health care hospital Punjab, Pakistan for examination with suspicion from Ap...

The present study was carried out to detect the circulating Newcastle disease virus (NDV) from various field outbreaks in Assam during 2018-2021. Nine hundred ninety-two clinical and post mortem samples were collected backyard commercial poultry subjected molecular detection by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). NDV could be detected 445 (n=757, 58.78%) tissue 53 (n=235, ...

List of non-standard abbreviations ATSDR Agency for Toxic Substances and Disease Registry GSK-3β Glycogen synthase kinase-3β Kim-1 Kidney injury molecule-1 RT-PCR Reverse transcriptase polymerase chain reaction

A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for human enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) infection was further evaluated. The one step reaction was performed in a single tube at 65 ̊C for 45 min for EV71 and 35 min for CVA16. The detection limits of RT-LAMP assays for both EV71 and CVA16 were 0.1 of a 50% tissue culture infective dos...

Abstract: Potato virus Y a type species of the genus potyvirus infects several crops in the family solanaceae. PVY isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. The result of ELISA, Immunoblot electrophoresis and Immuno Capture Reverse Transcription Polymerase Chain Reaction   (IC-RT-PCR) indicated that...

The major bcr-abl fusion gene is presently seen as the hallmark of chronic myeloid leukemia (CML) and presumably as the cause of its development. Accordingly, long-term disappearance of bcr-abl after intensive therapy is considered to be a probable cure of CML. The nested reverse transcriptase-polymerase chain reaction (RT-PCR) provides a powerful tool for minimal residual CML detection. The RT...

Dengue virus (DNV) is the cause of dengue infections and Pakistan along with the world is now being overloaded with their patients. The molecular epidemiological studies reveal that there are four serotypes of the DNV circulating throughout the endemic countries. Non-structural glycoprotein (NS-1) causes the damage of hepatocytes along with the activation of the complement system which in turn ...

This study was carried out in bean seeds Isparta and Antalya provinces to determine Bean common mosaic virus (BCMV) 2016. For this purpose; 106 seed samples were collected from the research area. Seed used for biological, serological, molecular methods. The incidence, as a percentage of BCMV, found 87.73% by DAS-ELISA 90.56% Immunocapture Reverse Transcriptase-Polymerase Chain Reaction (IC-RT-P...