This article describes reverse phase high-performance liquid chromatography (RPHPLC) methods for determination of diuretics in different human body fluids (whole blood, plasma, serum or urine). Sample preparation procedures, including solid-phase extraction, liquid-liquid extraction, dilution, precipitation as well as automated RPHPLC procedures, are discussed in order to present the advantages...

urinary 1-hydroxypuren (1-ohp) is commonly used as a major metabolite and biological indicator of the overall exposure to polycyclic aromatic hydrocarbons (pahs). for evaluation of human exposure to such compounds, biological monitoring is an essential process, in which, preparation of samples is one of the most time-consuming and error-prone aspects prior to chromatographic techniques. in this...

■ CONTENTS Principles of Single Molecule Spectroscopy and Instrumental Techniques 84 General Considerations and Sample Requirements 84 Confocal Microscopy 85 Total Internal Reflectance Fluorescence Wide Field Imaging and Analysis 86 Chromatography Methods Studied by Single Molecule Spectroscopy 87 Reverse Phase Liquid Chromatography 87 Silica Surfaces: Normal Phase Chromatography and Capillary ...