■ CONTENTS Principles of Single Molecule Spectroscopy and Instrumental Techniques 84 General Considerations and Sample Requirements 84 Confocal Microscopy 85 Total Internal Reflectance Fluorescence Wide Field Imaging and Analysis 86 Chromatography Methods Studied by Single Molecule Spectroscopy 87 Reverse Phase Liquid Chromatography 87 Silica Surfaces: Normal Phase Chromatography and Capillary ...

A system which consisted of multidimensional liquid chromatography (Yin-yang MDLC) coupled with mass spectrometry was used for the identification of peptides and phosphopeptides. The multidimensional liquid chromatography combines the strong-cation exchange (SCX), strong-anion exchange (SAX), and reverse-phase methods for the separation. Protein digests were first loaded on an SCX column. The f...

We present an HPLC method for the determination of amino acids in plasma. The method is based on automated precolumn derivatization of amino acids with o-phthalaldehyde, separation of the derivatives by reversed-phase chromatography, and quantification by fluorescence detection. Complete separation was achieved within 12 min. Total analysis time, including derivatization, chromatography, and re...

in published high performance liquid chromatographic (hplc) methods for analysis of topiramate (tpm) in pharmaceutical dosage forms and raw materials refractive index detector (rid) has been used which is not available in many laboratories and has low sensitivity. we described a new, sensitive and simple hplc method for determination of topiramate in pharmaceutical forms and in-virto dissolutio...

Garrido JL and Zapata M (1996) Ion-pair reversed-phase high-performance liquid chromatography of algal chlorophylls. Journal of Chromatography A 738: 285. Hodgson DA, Wright SW and Davies N (1997) Mass spectrometry and reverse phase HPLC techniques for the identiRcation of degraded fossil pigments in lake sediments and their application in palaeolimnology. Journal of Paleolimnology 18: 335. Jef...

Avian luteinizing hormone-releasing hormone (LHRH) has been isolated from 249,000 chicken hypothalami and shown to differ structurally from mammalian hypothalamic LH-RH. Purification was achieved by acetic acid extraction, anti-LH-RH affinity chromatography, and cation exchange and reverse phase high performance liquid chromatography. The isolated peptide eluted as a single peak on reverse phas...

background and objectives: analytical methods for volatile organic compounds (vocs) in different samples need extraction of compounds, by applying hazardous solvents. solid phase micro-extraction (spme) is a solvent-free equilibrium extraction method, in which proper calibration can allow quantitative determinations of vocs at a very good sensitivity without the use of any organic solvent. vocs...

background: determination of organic pollutants usually requires extraction of the pollutants from samples, using hazard­ous solvent. solid phase micro-extraction (spme) is a solvent-free equilibrium extraction method, in which, proper calibra­tion can allow quantitative measurements of organic pollutants at a very good sensitivity without the use of any organic sol­vent. because individual voc...