We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in </=6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLSI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomyc...

Comparisons of 2D and 3D cell culture models in literature have indicated differences in cellular morphology and metabolism, commonly attributed the better representation of in vivo conditions of the latter cell culture environment. Thus, interest in the use of 3D collagen gels for in vitro analysis has been growing. Although comparative studies to date have indicated an enhanced resistance of ...

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.

The CDC classifies Francisella tularensis as a Category A bioterrorism agent. Due to the risk of potential release antibiotic-resistant F. strains, new therapeutics against must be developed. Resazomycins are resazurin (Rz)-based compounds that exhibit antimicrobial activity and other gram-negative bacteria. mode action resazomycins is not understood, but targets antibiotic were identified in h...

Francisella tularensis is defined by the Centers for Disease Control and Prevention as a Category A bioterrorism agent due to its low infectious dose, high mortality rate, ease of aerosolization. Given there no licensed tularemia vaccine in United States possible development release antibiotic-resistant F. strains, an urgent need new treatments against this bacterium. We determined phenoxazine ...

Tularemia is a potentially fatal disease caused by the Category A bioterrorism agent Francisella tularensis. Aminoglycosides, fluoroquinolones, and tetracyclines can be used to treat tularemia; however, there high incidence of relapse treatment failures when using these drugs. Furthermore, no tularemia vaccine licensed for use in United States. Therefore, new antibiotics that target F. tularens...