To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. ...

Results Materials and Methods Four commercial dyes were used — Remazol® Black BA, Levafix® Blue Ca, Levafix® Yellow CA and Levafix® Red CA (Dystar). A standard anthraquinone dye, Remazol Brilliant Blue R (RBBR, Sigma) was also used. Dye stock solutions were added to solid or liquid decolorization media, up to 200 mg.L-1 final concentration. Rose Bengal Agar and Potato Dextrose Agar (both from D...

A magnetic Schiff’s base chitosan-glutaraldehyde/Fe3O4 composite (CHT-GLA/ZnO/Fe3O4) was developed by incorporating zinc oxide (ZnO) nanoparticles into its structure to prepare an efficient adsorbent for the removal of remazol brilliant blue R (RBBR) dye. The CHT-GLA/ZnO/Fe3O4 characterized following methods: CHN, BET, FTIR, XRD, SEM–EDX, pHpzc, and potentiometric titrations. Box-Behnken design...

Initial evaluations of large microbial libraries for potential producers of novel antimicrobial proteins require both qualitative and quantitative methods to screen for target enzymes prior to investing greater research effort and resources. The goal of this protocol is to demonstrate two complementary assays for conducting these initial evaluations. The microslide diffusion assay provides an i...