The objectives of this study were to evaluate different analytical methods determine colostrum quality in dairy cattle, including one laboratory-based method (ELISA) and 4 on-farm tests. We hypothesized that the colostral immunoglobulin G (IgG) concentration using methods, i.e., ELISA (mg/mL), digital Brix refractometer (% Brix), colostrometer (specific gravity mg/mL), an outflow funnel (second...