The main objective of this work is quantitative evaluation of porosity in carbon fiber reinforced polymers (CFRP) with the help of X-ray computed tomography (XCT). For this purpose two types of sample were compared. Industrial porosity sample was obtained from an aeronautical industry. Artificially induced porosity samples were created by drilling holes with diameter 0.2 mm and 0.3 mm in non-po...

Dispersive Liquid-Liquid Microextraction / Thermal Lens Microscopy (DLLME/TLM) was developed as a new combination method for preconcentration and determination of Cd. Thermal Lens Microscopy is suitable for the determination of analyte after DLLME because of the low volume of the remained phase after DLLME and increasing of the enhancement factor for the nonpolar organic solvents. Some effe...

Background: Malaria is still account for 200 million cases annually. Microscopy is the gold standard technique for malaria parasites detection. PCR-based techniques can detect malaria infections with high sensitivity. The study aimed to evaluate the sensitivity of microscopy technique in the detection of P. falciparum and P. vivax, using monoplex real-time PCR, Gezira State, Central Sudan. Met...

background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

background: candida albicans (c. albicans) is a major cause of candidaemia in people with impaired immunity. blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. we established a real-time pcr assay for c. albicans detection in blood by lightcycler pcr and melting curve analysis. methods: five milliliter blood samples from healthy volunt...

BACKGROUND A national multimedia campaign was launched in January 2010, to increase the proportion of young people tested for chlamydia. This study aimed to evaluate the impact of the campaign on the coverage and positivity within the National Chlamydia Screening Programme (NSCP) in England. METHOD An interrupted time series of anonymised NCSP testing reports for England for a 27 month period...

background:accumulative research is in progress to clarify clinical aspects of gbv-c. the possibility of interaction between hcv and gbv-c as well as its consequence on development of liver diseases is the most important clinical aspect which encourages researchers to develop a rapid and cost effective technique for simultaneous detection of both viruses.methods: in this study, a sybr green rea...

objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...