Influenza viral passaging through pre-vaccinated mice shows that emergent antigenic site mutations on the viral hemagglutinin (HA) impact host receptor-binding affinity and, therefore, the evolution of fitter influenza strains. To understand this phenomenon, we computed the Significant Interactions Network (SIN) for each residue and mapped the networks of antigenic site residues on a representa...

Ten previously reported lactose-positive (Lac) transconjugants from Streptococcus lactis, S. cremoris, and S. lactis subsp. diacetylactis and one sucrose-positive (Suc) transconjugant from S. lactis were examined for their sensitivity to prolate- and small isometric-headed bacteriophages. Four of the Lac transconjugants showed a 10- to 100-fold reduction in the efficiency of plating (EOP) as we...

BACKGROUND Red breast syndrome (RBS) has been described as an erythema that may be associated with 2-stage prosthetic reconstructive breast surgery using biologic mesh. RBS is differentiated from infectious cellulitis through absence of fever and laboratory abnormalities and usually has a self-limiting course. There have been no clinical reports on etiology, risk factors, or management of RBS. ...

Synthetic DNA duplexes corresponding to the ribosome binding site (RBS) were synthesized through the phosphite method on solid support. The synthetic RBS DNA with partial random sequences was inserted into an appropriate site between the lpp-lac promoter and the beta-galactosidase structural gene in plasmid pMKT2. The level of beta-galactosidase expression was correlated with the color intensit...

The actions of an intracellular nitric oxide generator compound on the properties of a co-culture model of the blood-brain barrier are described. Addition of the iron-sulphur cluster nitrosyl Roussin's black salt (RBS, heptanitrosyl-tri-mu3-thioxotetraferrate (1-)) resulted in a rapid and dose-dependent (50-250 microM) decline in the electrical resistance displayed by co-cultures of vascular en...

Translation of the sigma factor RpoS is activated by DsrA, RprA and ArcA, three small non-coding sRNAs (sRNA) that expose the ribosome-binding site (RBS) by opening up an inhibitory loop. In the RpoS network, no sRNAs have been found to pair with the RBS, a most common sRNA target site in bacteria. Here, we generate Ribo-0, an artificial sRNA, which represses rpoS translation by pairing with th...

Th e aim of this study was to establish the prevalence of repeat breeder syndrome (RBS) in diff erent breeds and reproductive ages of cattle in central Croatia and to assess the eff ectiveness of hormone therapy as a treatment option. A total of 1088 dairy cows were observed, 218 heifers and 870 multiparous cows. Aft er the third unsuccessful artifi cial insemination (n = 164) and a thorough cl...

This dissertation develops approaches to rapidly detect malicious network traffic including packets sent by portscanners and network worms. The main hypothesis is that stochastic models capturing a host’s particular connection-level behavior provide a good foundation for identifying malicious network activity in real-time. Using the models, the dissertation shows that a detection problem can be...

User association has emerged as a distributed resource allocation problem in the heterogeneous networks (HetNets). Although an approximate solution is obtainable using the approaches like combinatorial optimization and game theory-based schemes, these techniques can be easily trapped in local optima. Furthermore, the lack of exploring the relation between the quality of the solution and the par...