This study showed that vaccination of cattle with Brucella abortus rough strain RB51 induces incomplete antibodies that can be detectable by a Coombs antiglobulin test using the B. abortus 99 smooth strain.

conclusions purified lps can be considered as a safe adjuvant and can be used as a component in prophylactic and therapeutic vaccines targeting infectious disease, cancer and allergies. results biochemical analysis data and sds-page profile showed that the chemical nature of s19 lps is different from rb51 lps. both s and r-lps induce an immune response. t-cell immune response induced by both s ...

Studies of the serum bactericidal system in bovine brucellosis were undertaken to investigate the role of the humoral immune response in protection of cattle against the facultative intracellular parasite Brucella abortus. Fresh sera from normal control cattle, infected cattle, and cattle immunized with B. abortus cell envelopes were collected before treatment and during the course of immunizat...

bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reducedfertility in cows. since brucellosis eradication programme in iran uses vaccination, test, slaughter andquarantine as control measures, it is essential to distinguish vaccine strains from strains that cause infectionsamong vaccinated cattle herds. we developed and evaluated a multiplex polyme...

An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.

Brucella abortus Vaccine Strain RB51 Infection and Exposures Associated with Raw Milk Consumption — Wise County, Texas, 2017 Caitlin M. Cossaboom, DVM, PhD1,2; Grishma A. Kharod, MPH2; Johanna S. Salzer, DVM, PhD2; Rebekah V. Tiller, MPH2; Lindsay P. Campbell, PhD2; Karen Wu, MSPH2; María E. Negrón, DVM, PhD2; Naomi Ayala3; Nicole Evert, MS4; Jill Radowicz3; Jennifer Shuford, MD4; Shelley Stone...

Bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reducedfertility in cows. Since brucellosis eradication programme in Iran uses vaccination, test, slaughter andquarantine as control measures, it is essential to distinguish vaccine strains from strains that cause infectionsamong vaccinated cattle herds. We developed and evaluated a multiplex polyme...