نتایج جستجو برای: random mutagenesis

تعداد نتایج: 309957  

Journal: :Molecular bioSystems 2012
Xinhao Ye Chenming Zhang Y-H Percival Zhang

The Clostridium thermocellum cellobiose phosphorylase (CtCBP) is a large protein consisting of 812 amino acids and has great potential in the production of sugar phosphates, novel glycosides, and biofuels. It is relatively stable at 50 °C, but is rapidly inactivated at 70 °C. To stabilize CtCBP at elevated temperatures, two protein-engineering approaches were applied, i.e. site-directed mutagen...

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2014
Anke Bill Elizabeth M. Rosethorne Toby C. Kent Lindsay Fawcett Lynn Burchell Michiel T. van Diepen Anthony Marelli Sergey Batalov Loren Miraglia Anthony P. Orth Nicole A. Renaud Steven J. Charlton Martin Gosling L. Alex Gaither Paul J. Groot-Kormelink

The human prostacyclin receptor (hIP receptor) is a seven-transmembrane G protein-coupled receptor (GPCR) that plays a critical role in vascular smooth muscle relaxation and platelet aggregation. hIP receptor dysfunction has been implicated in numerous cardiovascular abnormalities, including myocardial infarction, hypertension, thrombosis and atherosclerosis. Genomic sequencing has discovered s...

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Journal: Iranian Biomedical Journal 2004
Abbas Tahzibi, Fatemeh Kamal, Mahnaz Mazaheri Assadi,

A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N¢-nitro-N-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on Siegmund-Wagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactan...

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Journal: :Journal of Biological Chemistry 2000

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Journal: :Infection and Immunity 1999

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Journal: :journal of physical & theoretical chemistry 2014
nastaran asghari moghaddam

p53 tumor suppressor gene, also known as “genome guardian” is mutated in more than half of allkind of cancers. in this study we have investigated the controls of environmental ph for p53 genemutation in point of specific sequence which is prone to mutagenesis. the most probable cancerousmutations occur as point mutations in exons 5-8 of p53 gene. the 175th codon of p53 is the thirdmost mutated ...

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ژورنال: مجله علوم پزشکی رازی 2003
آذرنوش, مرتضی, ساروخانی, محمد, معظمی, نسرین ,

The extent of cephalosporin C(CPC) production in producing strains is related with the expression or inhibition of the relevent genes. The goal of this study was manipulate of the producing strain in order to increase its CPC production. Acremonium chrysogenum DSM 2399 is used as parent strain. Mutagenesis is performed on solid form of optimized production media. UV ray and NTG (with ...

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Journal: :Applied and environmental microbiology 2006
Olga Monroy-Lagos Xavier Soberon Paul Gaytan Joel Osuna

Secretion of Escherichia coli penicillin acylase was improved by codon-based random mutagenesis of its signal peptide. The mutagenesis technology was applied to the gene region coding for positions Lys2 to Thr13 (N half) and Ala14 to Leu25 (C half) of the signal peptide. Protein secretion was higher in several signal peptide variants (up to fourfold with respect to the wild-type value).

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Journal: :Journal of Visualized Experiments 2016

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Journal: :Molecular Endocrinology 1987

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نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید

function paginate(evt) { url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term pg=parseInt(evt.target.text) var data={ "year":filter_year, "term":term, "pgn":pg } filtered_res=post_and_fetch(data,url) window.scrollTo(0,0); } function update_search_meta(search_meta) { meta_place=document.getElementById("search_meta_data") term=search_meta.term active_pgn=search_meta.pgn num_res=search_meta.num_res num_pages=search_meta.num_pages year=search_meta.year meta_place.dataset.term=term meta_place.dataset.page=active_pgn meta_place.dataset.num_res=num_res meta_place.dataset.num_pages=num_pages meta_place.dataset.year=year document.getElementById("num_result_place").innerHTML=num_res if (year !== "unfilter"){ document.getElementById("year_filter_label").style="display:inline;" document.getElementById("year_filter_place").innerHTML=year }else { document.getElementById("year_filter_label").style="display:none;" document.getElementById("year_filter_place").innerHTML="" } } function update_pagination() { search_meta_place=document.getElementById('search_meta_data') num_pages=search_meta_place.dataset.num_pages; active_pgn=parseInt(search_meta_place.dataset.page); document.getElementById("pgn-ul").innerHTML=""; pgn_html=""; for (i = 1; i <= num_pages; i++){ if (i===active_pgn){ actv="active" }else {actv=""} pgn_li="
  • " +i+ "
    • "; pgn_html+=pgn_li; } document.getElementById("pgn-ul").innerHTML=pgn_html var pgn_links = document.querySelectorAll('.mypgn'); pgn_links.forEach(function(pgn_link) { pgn_link.addEventListener('click', paginate) }) } function post_and_fetch(data,url) { showLoading() xhr = new XMLHttpRequest(); xhr.open('POST', url, true); xhr.setRequestHeader('Content-Type', 'application/json; charset=UTF-8'); xhr.onreadystatechange = function() { if (xhr.readyState === 4 && xhr.status === 200) { var resp = xhr.responseText; resp_json=JSON.parse(resp) resp_place = document.getElementById("search_result_div") resp_place.innerHTML = resp_json['results'] search_meta = resp_json['meta'] update_search_meta(search_meta) update_pagination() hideLoading() } }; xhr.send(JSON.stringify(data)); } function unfilter() { url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term var data={ "year":"unfilter", "term":term, "pgn":1 } filtered_res=post_and_fetch(data,url) } function deactivate_all_bars(){ var yrchart = document.querySelectorAll('.ct-bar'); yrchart.forEach(function(bar) { bar.dataset.active = false bar.style = "stroke:#71a3c5;" }) } year_chart.on("created", function() { var yrchart = document.querySelectorAll('.ct-bar'); yrchart.forEach(function(check) { check.addEventListener('click', checkIndex); }) }); function checkIndex(event) { var yrchart = document.querySelectorAll('.ct-bar'); var year_bar = event.target if (year_bar.dataset.active == "true") { unfilter_res = unfilter() year_bar.dataset.active = false year_bar.style = "stroke:#1d2b3699;" } else { deactivate_all_bars() year_bar.dataset.active = true year_bar.style = "stroke:#e56f6f;" filter_year = chart_data['labels'][Array.from(yrchart).indexOf(year_bar)] url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term var data={ "year":filter_year, "term":term, "pgn":1 } filtered_res=post_and_fetch(data,url) } } function showLoading() { document.getElementById("loading").style.display = "block"; setTimeout(hideLoading, 10000); // 10 seconds } function hideLoading() { document.getElementById("loading").style.display = "none"; } -->