The gene encoding for acetylxylan esterase from Bacillus pumilus has been cloned and expressed in Escherichia coli. The recombinant protein has been purified to homogeneity and crystallized. The crystals obtained are of regular shape of dimensions 0.05 x 0.05 x 0.05 mm with R32 symmetry and diffract to 2.0 A using synchrotron radiation.

A compressor was developed using R32 which was a low GWP R32 refrigerant for coping with global warming. However, there is a tendency for vibrations of a compressor to also increase because the circulation of refrigerant increases according to specification for using R32 refrigerant. Since large vibrations of a compressor causes outdoor units to generate solid propagation sound, there is a need...

The utilization of condensate water as a compressor-discharge cooler results in subcooling on the condenser outlet. On other hand, split-type air conditioner (A/C) with R32 working fluid can provide higher temperatures than refrigerants used same A/C. Therefore, A/C R32, equipped heat exchanger by utilizing waste-condensate cooler, has promising potential to produce largest effect air-condition...

Background: Alternative gene transcript splicing permits a single gene to produce multiple proteins with varied functions. Bioinformatic investigations have identified numerous splice variants, but whether these transcripts are translated to functional proteins and the physiological significance of these alternative proteins are largely unknown. Through direct identification of splice variants ...

Cocrystals of acemetacin drug (ACM) with nicotinamide (NAM), p-aminobenzoic acid (PABA), valerolactam (VLM) and 2-pyridone (2HP) were prepared by melt crystallization and their X-ray crystal structures determined by high-resolution powder X-ray diffraction. The powerful technique of structure determination from powder data (SDPD) provided details of molecular packing and hydrogen bonding in pha...

Bacterial Hfq is a protein that plays an important role in the regulation of genes in cooperation with sRNAs. Escherichia coli Hfq (EcHfq) has two or more sites that bind RNA(s) including U-rich and/or the poly(A) tail of mRNA. However, functional and structural information about Bacillus subtilis Hfq (BsHfq) including the RNA sequences that specifically bind to it remain unknown. Here, we desc...