nf-κb pathway is a link between inflammation and cancer and is involved in cellular responses to different stimuli. gastrointestinal lumen is exposed to many inflammatory agents such as foods, free radicals and bacterial or viral antigens. the aim of the present study was to evaluate the possible role of nf-κb1in gastric adenocarcinoma. to detect the relative level of nf-κb1transcript, total rn...

BACKGROUND Failure to early detect multidrug-resistant tuberculosis (MDR-TB) results in treatment failure and poor clinical outcomes, and highlights the need to rapidly detect resistance to rifampicin (RIF) and isoniazid (INH). METHODS In Multi-Fluorescence quantitative Real-Time PCR (MF-qRT-PCR) assay, 10 probes labeled with four kinds of fluorophores were designed to detect the mutations in...

PURPOSE Nodal micrometastasis and circulating tumor cells detected by multimarker quantitative real-time reverse transcription-PCR (qRT-PCR) may have prognostic importance in patients with colorectal cancer. EXPERIMENTAL DESIGN Paraffin-embedded sentinel lymph nodes from 67 patients and blood from 34 of these patients were evaluated in a prospective multicenter trial of sentinel lymph node ma...

Our laboratory has previously developed a qRT-PCR assay to assess drug efficacy on the growth of Cryptosporidium parvum in vitro by detecting the levels of parasite 18S rRNA. This approach displayed up to four orders of magnitude of linear dynamic range and was much less labor-intensive than the traditional microscopic methods. However, conventional qRT-PCR protocol is not very amendable to hig...

BACKGROUND The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both d...

BACKGROUND Real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for detection of carcinoembryonic antigen (CEA) mRNA in the peritoneal lavage of gastric cancer patients is now recognized as a useful method for the prediction of peritoneal recurrence after curative surgery. One problem with this method is that it is time-consuming and difficult to perform an intraoper...

qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for t...