نتایج جستجو برای: qpcr
تعداد نتایج: 13261 فیلتر نتایج به سال:
Since the invention of real-time PCR (qPCR), thousands of high-impact studies have been conducted and published using qPCR technique (Heid et al. 1996; Higuchi et al. 1993; VanGuilder, Vrana, and Freeman 2008). Because it is highly sensitive, qPCR is the preferred method for microarray data validation (Canales et al. 2006); however the most exciting applications have been in the discovery of ne...
زمینه و هدف: میکرو rnaهای موجود در گردش خون بیومارکرهایی امیدبخش برای تشخیص و ارزیابی بیماران مبتلا به سرطان می باشد. آزمون واکنش زنجیره ای پلی مراز کمی زمان واقعی(rt-qpcr) روشی حساس برای برآورد میزان بیان میکرو rna ها است. با این وجود، در حال حاضر هیچ توافقی برای انتخاب ژن های رفرنس مناسب برای آنالیزهای qpcr بر روی میکرو rna در گردش خون وجود ندارد. از این رو، هدف از این مطالعه انتخاب ژن رفرنس...
background: type 1 diabetes (t1d) is caused by cell-mediated autoimmune attack on pancreatic beta-cells. previous studies highlight the role of micrornas (mirnas) in the pathogenesis of t1d. mirnas are small non-coding rnas involved in the regulation of gene expression post-transcriptionally. in this work, mir-18b was chosen and the differential expression of it was measured between t1d patient...
زمینه و هدف: سرطان سینه 10 درصد از کل سرطان های موجود در دنیا را شامل می گردد و از زنان مبتلا به انواع سرطان ها 30 درصد مبتلا به سرطان سینه هستند. rnaهای غیرکدکننده بلند(lncrna)، گروه جدیدی از ژن های شناخته شده در ژنوم انسان هستند که از بخش وسیعی از ژنوم یوکاریوت ها رونویسی شده و در تنظیم فرآیندهای متنوع زیستی نقش های مهمی ایفا می کنند. هدف از این مطالعه مقایسه میزان بیان lncrnaهای gas5 و neat1...
conclusions in conclusion, the data showed that high rate of females harbor these microorganisms in the genital microbiota. this finding suggests the importance of routine gynecological tests, mainly in pregnant females, during the second and third trimesters, and in asymptomatic females under infertility clinical investigation. furthermore, the application of molecular methods is suggested; si...
Quantitative PCR (qPCR) is more sensitive than microscopy for detecting Pneumocystis jirovecii in bronchoalveolar lavage (BAL) fluid. We therefore developed a qPCR assay and compared the results with those of a routine immunofluorescence assay (IFA) and clinical data. The assay included automated DNA extraction, amplification of the mitochondrial large-subunit rRNA gene and an internal control,...
BACKGROUND Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic range. The gold standard method for estimating DNA concentrations via qPCR is quantification cycle () standard curve quantification, which requires the time- and labor-intensive construction of a standard curve. In theory, the sha...
We developed two real-time quantitative PCR (qPCR) assays, targeting the 28S rRNA gene, for the diagnosis of zygomycosis caused by the most common, clinically significant Zygomycetes. The amplicons of the first qPCR assay (qPCR-1) from Rhizopus, Mucor, and Rhizomucor species were distinguished through melt curve analysis. The second qPCR assay (qPCR-2) detected Cunninghamella species using a di...
BACKGROUND The White spot syndrome virus (WSSV) and Penaeus stylirostris penstyldensovirus 1 (previously named Infectious hypodermal and hematopoietic necrosis virus-IHHNV) are two of the most important viral pathogens of penaeid shrimp. Different methods have been applied for diagnosis of these viruses, including Real-time PCR (qPCR) assays. A duplex qPCR method allows the simultaneous detecti...
Methods is indexed/abstracted in the following publications: Print Indexes: ASCA (Automatic Subject Citation Alert), BBCI (Biochemistry & Biophysics Citation Index), Biological Abstracts, Chemical Abstracts, Current
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