We have developed a single-tube nested real-time PCR (STN-RT PCR) assay using the repetitive, transposon-like element IS1111 as the DNA target to facilitate early diagnosis of acute Q fever. The use of our proposed diagnostic procedures, including IgM detection by serology and the STN-RT PCR assay, significantly increased the diagnostic sensitivity for Q fever to 78%, compared to 29% when serol...

background: q fever is a zoonotic disease caused bycoxiella burnetii, a species of bacteria that is distributedworldwide. in cattle, coxiella burnetii infections are generallyasymptomatic but can also be associated with reproductivedisorders. objectives: the aim of this study was to achievemolecular detection of coxiella burnetii in dairy bovine milkfarms using nested pcr in qom province, iran....

OBJECTIVE To assess quantitative real-time polymerase chain reaction (q-PCR) for the sputum smear diagnosis of pulmonary tuberculosis (PTB) in patients living with HIV/AIDS with a clinical suspicion of PTB. METHOD This is a prospective study to assess the accuracy of a diagnostic test, conducted on 140 sputum specimens from 140 patients living with HIV/AIDS with a clinical suspicion of PTB, a...

Synovial biomarkers are increasingly important in the development of novel therapeutic agents for treatment rheumatoid arthritis (RA). To identify correlating with changes clinical disease activity, real-time quantitative PCR (Q-PCR) was used to evaluate synovial gene expression after corticosteroids. Patients active RA received either oral prednisolone (n = 10, 60 mg daily first week and 40 se...

Q fever is a worldwide zoonotic infectious disease due to Coxiella burnetii. The clinical presentation may be acute (pneumonia and/or hepatitis) or chronic (most commonly endocarditis). Diagnosis mainly relies on serology and PCR. We therefore developed a quantitative real-time PCR. We first tested blindly its performance on various clinical samples and then, when thoroughly validated, we appli...

BACKGROUND HER-2/neu is a proto-oncogene that encodes a transmembrane tyrosine kinase growth factor which is crucial for stimulating growth and cellular motility. Overexpression of HER-2/neu is observed in 10-35% of human breast cancers and is associated with pathogenesis, prognosis as well as response to therapy. Given the imperative role of HER-2/neu overexpression in breast cancer, it is imp...

Introducción: La concentración e integridad del ADN libre circulante (ADN-lc) son biomarcadores de diagnóstico y pronóstico en múltiples padecimientos. Objetivo. Determinar la índice ADN-lc una muestra población general Jalisco, México. Método. Se analizaron muestras sangre periférica 117 personas procedentes Jalisco. El se cuantificó mediante fluorometría Qubit 2.0 amplificación por q-PCR ALU1...

BACKGROUND Serologic diagnosis is one of the most widely used diagnostic methods for Q fever, but the window period in antibody response of 2 to 3 weeks after symptom onset results in significant diagnostic delay. We investigated the diagnostic utility of Q fever PCR from formalin-fixed liver tissues in Q fever patients with acute hepatitis. METHODS We reviewed the clinical and laboratory dat...