In order to establish the most sensitive and specific method in the detection of Rickettsia tsutsugamushi in Leptotrombidium deliense , mice and patients, a comparative study on DNA extraction, PCR, hybridization and PCR-hybridization was conducted. The results showed that the sensitivity of PCR-hybridization was 10 times higher than that of PCR and 1000 times higher than that of hybridization....

Stand problems consistently cause significant production losses and management problems in Arkansas rice fields. Previous research, funded by the Rice Research and Promotion Board, identified the role of environmental factors and soilborne plant pathogens in limiting rice stand establishment, and had the goal of determining the conditions where soilborne pathogens, especially Pythium species, p...

A DNA array containing 172 oligonucleotides complementary to specific diagnostic regions of internal transcribed spacers (ITS) of more than 100 species was developed for identification and detection of Pythium species. All of the species studied, with the exception of Pythium ostracodes, exhibited a positive hybridization reaction with at least one corresponding species-specific oligonucleotide...

The author investigated aquatic fungi developing on the seeds of some plants in various types of water bodies of Podlasie Province. The bait method was used to isolate the fungi. Seventy-four aquatic fungal species were found on seeds of three species of plants: Cannabis sativa hemp-seeds 59, Fagopyrum esculentum buckwheat-seeds 55 and on Vicia sativa vetch-seeds 44 in water of four various bod...

Damping-off diseases incited by different species of Pythium are a persistent problem worldwide, often resulting in reduced yields and occasionally resulting in major crop damage. There have been increasing restrictions on the use of chemical fungicides, and the development of disease-suppressive biocontrol agents has become a major goal of horticulture crops. An experiment was conducted with t...

ABSTRACT A series of laboratory tests were conducted to investigate potential effects of fungus gnat (Bradysia impatiens) feeding damage on susceptibility of geranium seedlings (Pelargonium x hortorum) to infection by the root rot pathogen Pythium aphanidermatum. Effects were compared with those from similar tests in which the seedlings were mechanically wounded by severing the root tip with a ...

Burkholderia cepacia AMMDR1 is a biocontrol agent that protects pea and sweet corn seeds from Pythium damping-off in field experiments. The goal of this work was to understand the effect of B. cepacia AMMDR1 on Pythium aphanidermatum and Aphanomyces euteiches zoospore homing events and on infection of pea seeds or roots. In vitro, B. cepacia AMMDR1 caused zoospore lysis, prevented cyst germinat...

Diagnosis of Pythium keratitis is problematic due to the difficulty in obtaining a culture report resulting in unnecessarily prolonged usage of antimicrobial medication due to misdiagnosis. This study evaluated and compared nested PCR technique with culture and immunoperoxidase staining assays of Pythium insidiosum in paraffin-embedded corneal tissues from patients with suspected fungal keratit...

We describe the ecological niche of the human and animal pathogen Pythium insidiosum within endemic agricultural areas of Thailand. Samples were collected from irrigation water, including rice paddy fields, irrigation channels and reservoirs. Zoospores of P. insidiosum were captured from water by the use of a sterile human hair baiting technique. Pythium isolates were identified based on phenot...

The influences of Gaeumannomyces graminis var. tritici (which causes take-all of wheat), Rhizoctonia solani AG-8 (which causes rhizoctonia root rot of wheat), Pythium irregulare, P. aristosporum, and P. ultimum var. sporangiiferum (which cause pythium root rot of wheat) on the population dynamics of Pseudomonas fluorescens 2-79 and Q72a-80 (bicontrol strains active against take-all and pythium ...