Glucose utilization by extracts of Pseudomonas jhwrescens involves its oxidation to gluconate and 2-ketogluconate (1, 2) by cytochrome-linked dehydrogenases (1). 2-Ketogluconate is phosphorylated by an inducible kinase, which is distinct from gluconokinase, and 2-keto-6-phosphogluconate, the phosphorylation product, has been isolated and identified (3). The conversion of 2-keto-6-phosphoglucona...

A nonaqueous fractionation method to obtain highly purified mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were determined in the purified mesophyll chloroplast fractions which were prepared from leaves exposed to different light intensities. The role of pyruvate in the regulation of pyruvate,Pi dikinase in these ...

We presentevidencefor the utilityof an improvedassay for the activity of lactate dehydrogenase (EC 1.1.1.27) isoenzymes1 and 2 in serum, involvinginhibitionof the H-subunit of LD by pyruvate at pH 7.1. Resultscorrelatewell with the LD-1/total LD ratio as evaluated by immunologicalassay and, as an indexto infarct, the method is superiorto either the change in CK-MB activityor to the LD-1 activit...

treated tumor (center). The peak pyruvate signal has been scale to 1 and the origin of time (t=0) has been chosen to coincide with the peak of the pyruvate signal. The panel at right shows an axial proton image of the tumor (indicated by the green ellipse) and the location of the surface coil (indicated by the red line). The diagonal line at upper left is a water-filled fiducial marker. Monitor...

Both assays are accepted reference methods for measurement of lactate dehydrogenase (LDH) activity (2-4). At pH 7.4, pyruvate -* lactate (P -* L) activity is about three times lactate -+ pyruvate (L P) activity, because equilibrium favors formation of lactate. Normal range for the P -* L assay is 130-265 spectrophotometric units at 25#{176} (3); normal range for the L P assay is 40-98 units at ...

Hepatocyte cultures derived from 15-day foetal rats produce the liver form of pyruvate kinase (EC 2.7.1.40) only after 3 days of culture. The appearance of the liver form of the enzyme can be blocked by the addition of 5-bromodeoxyuridine on day 2 of culture, but not by addition on day 3 of culture. The reversibility of the action of 5-bromodeoxyuridine was shown when the inhibitor was added on...

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