Abstract We analyzed the genome sequence of a Japanese pear (Pyrus pyrifolia) to facilitate its genetics and genomics as well breeding programs, in which variety 'Nijisseiki' with superior flesh texture has been used parent for most cultivars. De novo assembly long reads covered 136× generated 503.9 Mb contigs consisting 114 sequences an N50 value 7.6 Mb. Contigs were assigned genetic maps esta...