two experiments were performed for in vitro establishment, proliferation and shoot growth in axillary bud explants of sebri pear cultivar. in the first experiment, the effect of different concentrations of iba(indole-3-butyric acid) on explants’ establishment was evaluated. in a second experiment, the influence of several combinations of pgrs (plant growth regulators)on shoot proliferation and ...

Prunus and Pyrus species affected with phytoplasma diseases, as well as leafhopper species collected from Prunus and Pyrus fields in Ontario, Canada were tested for presence of phytoplasmas. Preliminary results showed that Graminella nigrifrons is a potential vector for phytoplasma groups 16SrI-W (‘Candidatus Phytoplasma asteris’), and 16SrVII-A (‘Candidatus Phytoplasma fraxini’) to a variety o...

Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar ‘Fertility’ by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morpholog...

total flavonoids contents and antibacterial activity of the extracts from the aerial parts of two labiateae species, nepeta menthoides boiss. & buhse and thymus trautvetteri klokov & desj.-shost. were determined. quantitative determination of flavonoid contents was calculated in terms of quercetin equivalent in various extracts (50% methanol, chloroform and distilled water) by alcl3 colorimetri...

The synthesis of semiconductor nanoparticles is an expanding research area due to the potential applications in the development of nanotechnologies. Especially, biosynthesized nanoparticles have become an important branch of nanotechnology. In the present work, we describe a low-cost and simple procedure for biosynthesis of Zinc oxide nanoparticles (ZnO NPs) using Pyrus Pyrifolia leaf extract. ...

We developed retrotransposon-based insertional polymorphism (RBIP) markers based on the long terminal repeat (LTR) sequences of copia-like retrotransposon Ppcrt4 and flanking genome sequences, which were derived from 454 sequencing data from Japanese pear (Pyrus pyrifolia) 'Hosui'. Out of 40 sequences including both LTR and flanking genome regions, we developed 22 RBIP markers and used them for...