The biochemical properties of polyamine transport system have been studied in detail in NB-15 mouse neuroblastoma cells in culture by measuring the uptake of [14C]putrescine under various experimentally imposed pharmacological conditions. Putrescine uptake in the NB-15 mouse neuroblastoma cells appeared to be a sodium-dependent process. Iso-osmotic displacement of Na+ in the assay medium with e...

BACKGROUND The polyamines (putrescine, spermidine, and spermine) are involved in cellular growth, proliferation, and differentiation. In the lungs of various species, polyamines are accumulated by an active uptake system which also mediates the uptake of cystamine and paraquat. In the rat lung putrescine uptake has been shown to be cell-specific, occurring predominantly in the alveolar epitheli...

in this research, we studied polyamines including putrescine (put), spermidine (spd) and spermine (spm) changes in three common bean (phaseolus vulgaris) genotypes, k-s-31167, ge-288 and naz under drought stress. our result showed steadily increase in putrescine (put) and spermine (spm) in all three genotypes.  ge-288 genotype showed decrease and then increasing in spermidine (spd) content but ...

Interactions between absorption of paraquat and the polyamines putrescine, cadaverine, and spermine in roots of intact maize (Zea mays L. cv 3377 Pioneer) seedlings were examined. Concentration-dependent kinetics for paraquat and putrescine influx were similar and both kinetic curves could be resolved into a linear and a saturable component. The linear component was previously shown to represen...

Putrescine (1,4-diaminobutane), the product of the decarboxylation of ornithine, is a precursor of the polyamines spermidine and spermine. After administration in uiuo of radiolabeled ornithine or putrescine, changes in specific activity of putrescine, spermidine, and spermine have been assessed in normal and regenerating liver and in brain. In both normal liver and in brain, after labeling wit...

softening and microbial activity are the most common problems of kiwifruit during storage. in order to improve these problems, pre- harvest treatments of putrescine and spermidine were studied on shelf life of kiwifruit cv. hayward. foliar spraying of different concentrations of purescine (0, 1 and 2 mm) and spermidine (0, 1 and 2 mm) has been done at 40 and 20 days before harvest. different ch...

An improved method of assay of urinary polyamines (putrescine, spermidine, and spermine) was applied to the study of cancer patients and an experimental gastric tumor of rats. Although total polyamines (putrescine, spermidine, and spermine) in urine of patients with blood and solid cancers were significantly high, putrescine concentrations also increased significantly and were shown to be of di...

Germination is considered a critical step in the development cycle of plant. But no information available regarding seed priming with Putrescine, Jasmonic acid, Kinetin, Potassium Nitrate (KNO3), Salicylic acid at time aged and non-aged seeds sorghum cultivars. However, to know effect aforesaid chemicals cultivars on germination rate, vigour alpha-amylase activity, research was conducted under ...

objective: in order to investigate effects of different concentration of gibberlic acid and poly amines on germination and early growth of pishtaz cultivar under salinity stress. methods: the experiment was carried out as factorial based on completely randomized design with four replicates in laboratory of agronomy and plant breeding of agricultural department, islamic azad university, varamin-...

The aim of this study was to investigate the effect of cell spreading on the induction of ornithine decarboxylase and the rate of putrescine uptake in anchorage-dependent and anchorage-independent cells. Plating non-transformed IEC-6 epithelial cells at high versus low cell density restricted cell spreading from 900 microns 2 to approximately 140 microns 2, blunted the transient induction of or...